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Literature summary extracted from

  • Ferrara, M.C.; Cobucci-Ponzano, B.; Carpentieri, A.; Henrissat, B.; Rossi, M.; Amoresano, A.; Moracci, M.
    The identification and molecular characterization of the first archaeal bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase demonstrate that family GH116 is made of three functionally distinct subfamilies (2014), Biochim. Biophys. Acta, 1840, 367-377.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Saccharolobus solfataricus
3.2.1.52 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 (2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol
-
 Saccharolobus solfataricus
3.2.1.21 conduritol beta-epoxide
-
 Saccharolobus solfataricus
3.2.1.21 N-butyldeoxynojirimycin
-
 Saccharolobus solfataricus
3.2.1.52 (2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol
-
 Saccharolobus solfataricus
3.2.1.52 conduritol beta-epoxide
-
 Saccharolobus solfataricus
3.2.1.52 N-butyldeoxynojirimycin
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.8
-
 4-nitrophenyl beta-D-glucoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 0.9
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 2.1
-
 cellobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 10.8
-
 N,N'-diacetyl-beta-chitobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 0.8
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 0.9
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 1.1
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 4.15
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 4.15
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 93400
-
 3 * 93400, SDS-PAGE Saccharolobus solfataricus
3.2.1.21 93418
-
 3 * 93418, calculated from sequence Saccharolobus solfataricus
3.2.1.21 271000
-
 gel filtration Saccharolobus solfataricus
3.2.1.21 313000
-
 gel filtration Saccharolobus solfataricus
3.2.1.52 93400
-
 3 * 93400, SDS-PAGE Saccharolobus solfataricus
3.2.1.52 93418
-
 3 * 93418, calculated from sequence Saccharolobus solfataricus
3.2.1.52 313000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Saccharolobus solfataricus Q97UH8
-
-
3.2.1.21 Saccharolobus solfataricus P2 Q97UH8
-
-
3.2.1.52 Saccharolobus solfataricus Q97UH8
-
-
3.2.1.52 Saccharolobus solfataricus P2 Q97UH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21
-
 Saccharolobus solfataricus
3.2.1.52
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 7.5
-
 pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 7.5
-
 pH 4.0, 60°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus P2 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl cellobioside + H2O
-
 Saccharolobus solfataricus beta-D-glucose + ?
-
 ?
3.2.1.21 2-nitrophenyl cellobioside + H2O
-
 Saccharolobus solfataricus P2 beta-D-glucose + ?
-
 ?
3.2.1.21 4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl N,N'-diacetyl-beta-chitobioside + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.21 cellobiose + H2O
-
 Saccharolobus solfataricus 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Saccharolobus solfataricus P2 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Saccharolobus solfataricus beta-D-glucose + ?
-
 ?
3.2.1.21 cellotetraose + H2O
-
 Saccharolobus solfataricus beta-D-glucose + ?
-
 ?
3.2.1.21 N,N'-diacetyl-beta-chitobiose + H2O
-
 Saccharolobus solfataricus N-acetyl-D-glucosamine
-
 ?
3.2.1.52 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus beta-D-galactose + 2-nitrophenol
-
 ?
3.2.1.52 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus beta-D-glucose + 2-nitrophenol
-
 ?
3.2.1.52 4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus beta-D-glucose + 4-methylumbelliferone
-
 ?
3.2.1.52 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
 Saccharolobus solfataricus N-acetyl-beta-D-glucosamine + 4-methylumbelliferone
-
 ?
3.2.1.52 4-nitrophenyl beta-D-galactose + H2O
-
 Saccharolobus solfataricus beta-D-galactose + 4-nitrophenol
-
 ?
3.2.1.52 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus beta-D-glucose + 4-nitrophenol
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Saccharolobus solfataricus N-acetyl-beta-D-glucosamine + 4-nitrophenol
-
 ?
3.2.1.52 GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O
-
 Saccharolobus solfataricus GlcNAc + ?
-
 ?
3.2.1.52 GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O
-
 Saccharolobus solfataricus GlcNAc + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 trimer 3 * 93400, SDS-PAGE Saccharolobus solfataricus
3.2.1.21 trimer 3 * 93418, calculated from sequence Saccharolobus solfataricus
3.2.1.52 trimer 3 * 93400, SDS-PAGE Saccharolobus solfataricus
3.2.1.52 trimer 3 * 93418, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase
-
 Saccharolobus solfataricus
3.2.1.21 SSO3039
-
 Saccharolobus solfataricus
3.2.1.52 bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase
-
 Saccharolobus solfataricus
3.2.1.52 SSO3039
-
 Saccharolobus solfataricus
3.2.1.52 SSO3039 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
 assay at Saccharolobus solfataricus
3.2.1.21 70
-
-
 Saccharolobus solfataricus
3.2.1.52 60
-
 assay at Saccharolobus solfataricus
3.2.1.52 70
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 65
-
 stable for 24 h, about 20% loss of activity after 48 h Saccharolobus solfataricus
3.2.1.21 85
-
 about 45% loss of activity after 8 h, about 60% loss of activity after 24 h Saccharolobus solfataricus
3.2.1.52 65
-
 stable for 24 h, about 20% loss of activity after 48 h Saccharolobus solfataricus
3.2.1.52 85
-
 about 45% loss of activity after 8 h, about 60% loss of activity after 24 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.56
-
 N,N'-diacetyl-beta-chitobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 1.8
-
 cellobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 8.8
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 9.15
-
 4-nitrophenyl beta-D-glucoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 5.4
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 8.8
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 9.15
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 19.2
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 19.2
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4
-
-
 Saccharolobus solfataricus
3.2.1.52 4
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 5 pH 3.0: about 70% of maximal activity, pH 5.0: about 70% of maximal activity Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.00515
-
 (2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 9
-
 conduritol beta-epoxide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 0.00515
-
 (2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 9
-
 conduritol beta-epoxide pH 4.0, 60°C Saccharolobus solfataricus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.21 0.00515
-
 pH 7.0, 45°C Saccharolobus solfataricus N-butyldeoxynojirimycin
3.2.1.21 9
-
 pH 7.0, 45°C Saccharolobus solfataricus conduritol beta-epoxide
3.2.1.52 0.00515
-
 pH 7.0, 45°C Saccharolobus solfataricus N-butyldeoxynojirimycin
3.2.1.52 9
-
 pH 7.0, 45°C Saccharolobus solfataricus conduritol beta-epoxide

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 0.05
-
 N,N'-diacetyl-beta-chitobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 0.9
-
 cellobiose pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 9.9
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.21 11.4
-
 4-nitrophenyl beta-D-glucoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 1.6
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 1.6
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 4.9
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 9.9
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
3.2.1.52 11.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus