EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.21 | E695A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
1.11.1.21 | E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
1.11.1.21 | L690A/R691A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
1.11.1.21 | L690A/R691A/E695A/Y697A | substantial increase in hexa-coordinate low-spin heme and diminished enzyme activity, complete loss of the capacity for the reactivation of the N-terminal domain | Mycobacterium tuberculosis |
1.11.1.21 | R691A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
1.11.1.21 | Y697A | only subtle variations in spectroscopic and catalytic properties of the enzyme, substantial decrease in the rate and extent of KatG N-terminal domain reactivation | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 1.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2.4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 3.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 3.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 4.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.21 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.21 | 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycobacterium tuberculosis | ? + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 160 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1020 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1050 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 3500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 4000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 4800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 6800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 8300 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 8900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 11500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.21 | 70 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A/E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 600 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 800 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 850 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A/Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 900 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant L690A/R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 1700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant R691A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 2500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 3100 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant E695A, pH 7.0, 23°C | Mycobacterium tuberculosis | |
1.11.1.21 | 4400 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant Y697A, pH 7.0, 23°C | Mycobacterium tuberculosis |