Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Culbertson, J.E.; Toney, M.D.
    Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production (2013), Biochim. Biophys. Acta, 1834, 240-246.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.86
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.85 1-hydroxyphenazine inhibitory above 0.200 mM Pseudomonas aeruginosa
2.6.1.85 additional information enzyme is not regulated by feedback inhibition. Little or no inhibition up to 1 mM: glyphosate, pyridoxamine 5'-phosphate, pyridoxal 5'-phosphate, and 3-hydroxypyridine-4-carboxylic acid Pseudomonas aeruginosa
2.6.1.85 phosphate phosphate buffer competitively inhibits PhzE, about 20% inhibition with 100 mM potassium phosphate at pH 7.0 Pseudomonas aeruginosa
2.6.1.85 pyocyanin inhibitory above 0.250 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.85 0.02
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.85 0.216
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.85 11
-
 L-glutamine pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.86 0.02
-
 chorismate L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.85 Co2+
-
 Pseudomonas aeruginosa
2.6.1.85 Mg2+ required, Km value 0.29 mM Pseudomonas aeruginosa
2.6.1.85 Mn2+
-
 Pseudomonas aeruginosa
2.6.1.86 Mg2+ lower activity with Mn2+, Co2+, no detectable activity with Zn2+, Fe2+, Ca2+ Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.85 71251
-
-
 Pseudomonas aeruginosa
2.6.1.86 71251
-
 x * 71251, His-tagged PhzE, MALDI-TOF Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Pseudomonas aeruginosa Q7DC81
-
-
2.6.1.86 Pseudomonas aeruginosa G3XCK6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.86
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-glutamine
-
 Pseudomonas aeruginosa L-glutamate + 4-amino-4-deoxychorismate
-
 ?
2.6.1.85 chorismate + NH3
-
 Pseudomonas aeruginosa 4-amino-4-deoxychorismate + H2O
-
 ?
2.6.1.85 additional information isoform PhzE catalyzes the addition of ammonia to C2 of chorismate, as does anthranilate synthase, yet unlike anthranilate synthase it does not catalyze elimination of pyruvate from enzyme-bound aminodeoxyisochorismate. Reaction follows a random kinetic mechanism, enzyme PhzE can use NH4+ as an alternative nucleophile. PhzE shows no lyase activity Pseudomonas aeruginosa ?
-
 ?
2.6.1.86 L-glutamine + chorismate
-
 Pseudomonas aeruginosa (S)-2-amino-2-deoxyisochorismate + glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.85 ? x * 71274, calculated, x * 71251, MALDI-TOF, His-tagged recombinant protein Pseudomonas aeruginosa
2.6.1.86 ? x * 71251, His-tagged PhzE, MALDI-TOF Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.6.1.85 PhzE
-
 Pseudomonas aeruginosa
2.6.1.86 aminodeoxyisochorismate synthase
-
 Pseudomonas aeruginosa
2.6.1.86 PhzE
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.85 25 30
-
 Pseudomonas aeruginosa
2.6.1.86 25 30
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.85 45
-
 20% of maximum activtiy Pseudomonas aeruginosa
2.6.1.86 10 45
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.85 0.16
-
 chorismate cosubstrate NH3, pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.85 2.2
-
 chorismate cosubstrate L-glutamine, pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.86 0.16
-
 chorismate ammonium sulfate as ammonia source, pH 7.0, 25°C Pseudomonas aeruginosa
2.6.1.86 2.2
-
 chorismate L-glutamine as ammonia source, pH 7.0, 25°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.85 7
-
 assay at Pseudomonas aeruginosa
2.6.1.86 7
-
 optimal activity at low pH values between pH 6.5 and pH 8.5 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.86 6.5 8.5
-
 Pseudomonas aeruginosa