EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.18 | expressed in Aspergillus oryzae Jal355 cells | Thermothielavioides terrestris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.0039 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.0056 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.0075 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.0082 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.023 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.078 | - |
2,6-dichlorophenol-indophenol | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 2.5 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 54 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1000 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.18 | additional information | secretome | Thermothielavioides terrestris | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 125000 | - |
x * 125000, SDS-PAGE | Thermothielavioides terrestris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellopentaose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellopentaose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotetraose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotetraose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotriose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotriose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | D-xylose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | lactose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | maltose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | additional information | Thermothielavioides terrestris | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | ? | - |
? | |
1.1.99.18 | additional information | Thermothielavioides terrestris NRRL8126 | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Thermothielavioides terrestris | - |
- |
- |
1.1.99.18 | Thermothielavioides terrestris NRRL8126 | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.99.18 | glycoprotein | approximately 40% of the expressed and processed protein represents glycosylation | Thermothielavioides terrestris |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | Mono Q column chromatography and Source 15PHE column chromatography | Thermothielavioides terrestris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 0.85 | - |
purified enzyme, with cellobiose as substrate, in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellopentaose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellopentaose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotetraose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotetraose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotriose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | cellotriose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | D-xylose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | lactose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | maltose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
1.1.99.18 | additional information | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | ? | - |
? | |
1.1.99.18 | additional information | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.18 | ? | x * 125000, SDS-PAGE | Thermothielavioides terrestris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.18 | cbdA | - |
Thermothielavioides terrestris |
1.1.99.18 | Cdh | - |
Thermothielavioides terrestris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 65 | 87 | the enzyme unfolds over the range of 65-87°C, with a Tm value of 80.2°C | Thermothielavioides terrestris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.17 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.33 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.01 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.23 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.23 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.23 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.25 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 1.4 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 2.05 | - |
2,6-dichlorophenol-indophenol | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 6 | - |
- |
Thermothielavioides terrestris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4 | 6 | below pH 4.0, and particularly above pH 6.0, the activity is observed to drop (65% activity at pH 3.0, 45% activity at pH 7.0 and no activity at pH 9.0) | Thermothielavioides terrestris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | FAD | - |
Thermothielavioides terrestris | |
1.1.99.18 | heme | - |
Thermothielavioides terrestris |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.00033 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.019 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 0.068 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 62 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 150 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 160 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 220 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.1.99.18 | 320 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |