EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.237 | expression in Escherichia coli | Wickerhamia fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.237 | Cu2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
1.1.1.237 | Fe2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
1.1.1.237 | Hg2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
1.1.1.237 | WO42- | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
1.1.1.237 | Zn2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.237 | 0.01 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 0.1 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 0.4 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 0.64 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 3.5 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 18.9 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.237 | 40000 | - |
2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
1.1.1.237 | 40300 | - |
2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
1.1.1.237 | 75000 | - |
gel filtration | Wickerhamia fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.237 | Wickerhamia fluorescens | F1T2J9 | - |
- |
1.1.1.237 | Wickerhamia fluorescens TK1 | F1T2J9 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.237 | 727 | - |
pH 6.5, 25°C | Wickerhamia fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.237 | 4-hydroxyphenylpyruvate + NADPH + H+ | - |
Wickerhamia fluorescens | D-(4-hydroxyphenyl)lactate + NADP+ | - |
? | |
1.1.1.237 | 4-hydroxyphenylpyruvate + NADPH + H+ | - |
Wickerhamia fluorescens TK1 | D-(4-hydroxyphenyl)lactate + NADP+ | - |
? | |
1.1.1.237 | glyoxylate + NADPH + H+ | - |
Wickerhamia fluorescens | glycolate + NADP+ | - |
? | |
1.1.1.237 | glyoxylate + NADPH + H+ | - |
Wickerhamia fluorescens TK1 | glycolate + NADP+ | - |
? | |
1.1.1.237 | hydroxypyruvate + NADH + H+ | - |
Wickerhamia fluorescens | D-glycerate + NAD+ | - |
? | |
1.1.1.237 | hydroxypyruvate + NADH + H+ | - |
Wickerhamia fluorescens TK1 | D-glycerate + NAD+ | - |
? | |
1.1.1.237 | additional information | no substrates: pyruvate, oxaloacetate or benzoylformate | Wickerhamia fluorescens | ? | - |
? | |
1.1.1.237 | additional information | no substrates: pyruvate, oxaloacetate or benzoylformate | Wickerhamia fluorescens TK1 | ? | - |
? | |
1.1.1.237 | phenylpyruvate + NADPH + H+ | more than 99.9% D-isomer, L-isomer below limits of detection | Wickerhamia fluorescens | D-phenyllactate + NADP+ | - |
? | |
1.1.1.237 | phenylpyruvate + NADPH + H+ | more than 99.9% D-isomer, L-isomer below limits of detection | Wickerhamia fluorescens TK1 | D-phenyllactate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.237 | dimer | 2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.237 | Ppr | - |
Wickerhamia fluorescens |
1.1.1.237 | pprA | - |
Wickerhamia fluorescens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.237 | 9.1 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 19 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 31 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 73 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 121 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 150 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.237 | 6.5 | 7 | - |
Wickerhamia fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.237 | NAD+ | - |
Wickerhamia fluorescens | |
1.1.1.237 | NADH | NADPH is preferred over NADH | Wickerhamia fluorescens | |
1.1.1.237 | NADP+ | - |
Wickerhamia fluorescens | |
1.1.1.237 | NADPH | preferred over NADH | Wickerhamia fluorescens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.1.237 | Wickerhamia fluorescens | presence of phenylalanine results in up to 40fold increase in transcripts | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.237 | physiological function | Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA | Wickerhamia fluorescens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.237 | 1 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 2.6 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 110 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 310 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 380 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
1.1.1.237 | 12000 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens |