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Literature summary extracted from

  • Frasconi, M.; Favero, G.; Boer, H.; Koivula, A.; Mazzei, F.
    Kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin (2010), Biochim. Biophys. Acta, 1804, 899-908.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 enzyme expression in Trichoderma reesei under the strong cbh1 (cel7A) promoter in a strain in which the major cellulase gene cbh1 is disrupted Melanocarpus albomyces

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Trametes versicolor
1.10.3.2 additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Melanocarpus albomyces
1.10.3.2 additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Trametes hirsuta
1.10.3.2 additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Toxicodendron vernicifluum
1.10.3.2 0.0024
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 0.006
-
 dopamine pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 0.031
-
 catechol pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 0.034
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.041
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.045
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.066
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes versicolor
1.10.3.2 0.069
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.092
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes versicolor
1.10.3.2 0.098
-
 catechol pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.13
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes versicolor
1.10.3.2 0.16
-
 catechol pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.17
-
 dopamine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.28
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 0.29
-
 dopamine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.32
-
 ferrocyanide pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.38
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.41
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 0.45
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes hirsuta
1.10.3.2 0.79
-
 ferrocyanide pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.83
-
 ferrocyanide pH 5.0, 25°C Trametes versicolor
1.10.3.2 0.89
-
 ferrocyanide pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 1.11
-
 catechol pH 5.0, 25°C Trametes versicolor
1.10.3.2 2.1
-
 dopamine pH 5.0, 25°C Trametes versicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 55000
-
 x * 55000, native enzyme, SDS-PAGE Trametes hirsuta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 4 catechol + O2 Trametes versicolor
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Melanocarpus albomyces
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Trametes hirsuta
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Toxicodendron vernicifluum
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Trametes hirsuta VTT D-95443
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Melanocarpus albomyces
-
-
-
1.10.3.2 Toxicodendron vernicifluum Q8H979
-
-
1.10.3.2 Trametes hirsuta Q02497
-
-
1.10.3.2 Trametes hirsuta VTT D-95443 Q02497
-
-
1.10.3.2 Trametes versicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme from culture filtrate by anion exchange and hydrophobic interaction chromatography Trametes hirsuta
1.10.3.2 recombinant enzyme from a Trichoderma reeseicbh1 cbh1 disruption mutant strain Melanocarpus albomyces

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 commercial preparation
-
 Trametes versicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
-
 Melanocarpus albomyces ?
-
 ?
1.10.3.2 (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
-
 Trametes hirsuta VTT D-95443 ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Melanocarpus albomyces ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes hirsuta VTT D-95443 ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes versicolor 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Melanocarpus albomyces 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes hirsuta 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Toxicodendron vernicifluum 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes hirsuta VTT D-95443 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Trametes versicolor ?
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Melanocarpus albomyces ?
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Trametes hirsuta ?
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 dopamine + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 dopamine + O2
-
 Melanocarpus albomyces ?
-
 ?
1.10.3.2 dopamine + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 dopamine + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 dopamine + O2
-
 Trametes hirsuta VTT D-95443 ?
-
 ?
1.10.3.2 ferrocenemonocarboxylic acid + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 ferrocenemonocarboxylic acid + O2
-
 Melanocarpus albomyces ?
-
 ?
1.10.3.2 ferrocenemonocarboxylic acid + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 ferrocenemonocarboxylic acid + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 ferrocyanide + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 ferrocyanide + O2
-
 Melanocarpus albomyces ?
-
 ?
1.10.3.2 ferrocyanide + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 ferrocyanide + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 ferrocyanide + O2
-
 Trametes hirsuta VTT D-95443 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 55000, native enzyme, SDS-PAGE Trametes hirsuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Trametes versicolor
1.10.3.2 25
-
 assay at Melanocarpus albomyces
1.10.3.2 25
-
 assay at Trametes hirsuta
1.10.3.2 25
-
 assay at Toxicodendron vernicifluum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.1
-
 ferrocyanide pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.32
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 0.92
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 2.6
-
 catechol pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 4.4
-
 dopamine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 10.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 64
-
 ferrocyanide pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 75
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 77
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes versicolor
1.10.3.2 83
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes versicolor
1.10.3.2 88
-
 catechol pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 108
-
 ferrocyanide pH 5.0, 25°C Trametes hirsuta
1.10.3.2 109
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 115
-
 catechol pH 5.0, 25°C Trametes versicolor
1.10.3.2 116
-
 dopamine pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 122
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 130
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes versicolor
1.10.3.2 137
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes hirsuta
1.10.3.2 140
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 184
-
 dopamine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 186
-
 dopamine pH 5.0, 25°C Trametes versicolor
1.10.3.2 196
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes hirsuta
1.10.3.2 202
-
 catechol pH 5.0, 25°C Trametes hirsuta
1.10.3.2 215
-
 ferrocyanide pH 5.0, 25°C Trametes versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5 5 assay at Trametes versicolor
1.10.3.2 4.5 5 assay at Melanocarpus albomyces
1.10.3.2 4.5 5 assay at Trametes hirsuta
1.10.3.2 4.5 5 assay at Toxicodendron vernicifluum

General Information

EC Number General Information Comment Organism
1.10.3.2 additional information the laccase from Melanocarpus albomyces is a low redox potential enzyme. Structure-function study, mass spectrometry, overview Melanocarpus albomyces
1.10.3.2 additional information the laccase from Rhus vernicifera is a low redox potential enzyme. Structure-function study, mass spectrometry, overview Toxicodendron vernicifluum
1.10.3.2 additional information the laccase from Trametes hirsuta is a high redox potential enzyme. Structure-function study, mass spectrometry, overview Trametes hirsuta
1.10.3.2 additional information the laccase from Trametes versicolor is a high redox potential enzyme. Structure-function study, mass spectrometry, overview Trametes versicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.13
-
 ferrocyanide pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 2.3
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 4.3
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 25.1
-
 dopamine pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 26.5
-
 catechol pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 72
-
 ferrocyanide pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 88
-
 dopamine pH 5.0, 25°C Trametes versicolor
1.10.3.2 103
-
 catechol pH 5.0, 25°C Trametes versicolor
1.10.3.2 185
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 222
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Toxicodendron vernicifluum
1.10.3.2 259
-
 ferrocyanide pH 5.0, 25°C Trametes versicolor
1.10.3.2 306
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes hirsuta
1.10.3.2 334
-
 ferrocyanide pH 5.0, 25°C Trametes hirsuta
1.10.3.2 380
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 643
-
 dopamine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 956
-
 ferrocenemonocarboxylic acid pH 5.0, 25°C Trametes versicolor
1.10.3.2 1024
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes versicolor
1.10.3.2 1167
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes versicolor
1.10.3.2 1208
-
 dopamine pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 1232
-
 catechol pH 5.0, 25°C Trametes hirsuta
1.10.3.2 2838
-
 catechol pH 5.0, 25°C Melanocarpus albomyces
1.10.3.2 3288
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Trametes hirsuta
1.10.3.2 4780
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Trametes hirsuta
1.10.3.2 58330
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH 5.0, 25°C Melanocarpus albomyces