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Literature summary extracted from

  • Kim, J.; Copley, S.D.
    The orphan protein bis-?-glutamylcystine reductase joins the pyridine nucleotide disulfide reductase family (2013), Biochemistry, 52, 2905-2913.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.13
-
 Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.13 1.1
-
 bis-gamma-glutamylcystine pH 6.7, temperature not specified in the publication Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.13 Halobacterium salinarum Q9HSN0
-
-
1.8.1.13 Halobacterium salinarum NRC 1 Q9HSN0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.13 both native enzyme and recombinant enzyme, from inclusion bodies Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.13 bis-gamma-glutamylcystine + NADPH + H+
-
 Halobacterium salinarum 2 gamma-glutamylcysteine + NADP+
-
 ?
1.8.1.13 bis-gamma-glutamylcystine + NADPH + H+
-
 Halobacterium salinarum NRC 1 2 gamma-glutamylcysteine + NADP+
-
 ?
1.8.1.13 additional information enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity Halobacterium salinarum ?
-
 ?
1.8.1.13 additional information enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity Halobacterium salinarum NRC 1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.13 GCR
-
 Halobacterium salinarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.13 54
-
 bis-gamma-glutamylcystine pH 6.7, temperature not specified in the publication Halobacterium salinarum

General Information

EC Number General Information Comment Organism
1.8.1.13 physiological function enzyme is a member of the pyridine nucleotide disulfide oxidoreductase family Halobacterium salinarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.13 49
-
 bis-gamma-glutamylcystine pH 6.7, temperature not specified in the publication Halobacterium salinarum