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Literature summary extracted from

  • Herwald, S.; Liu, A.Y.; Zhu, B.E.; Sea, K.W.; Lopez, K.M.; Sazinsky, M.H.; Crane, E.J.
    Structure and substrate specificity of the pyrococcal coenzyme A disulfide reductases/polysulfide reductases (CoADR/Psr): implications for S(0)-based respiration and a sulfur-dependent antioxidant system in Pyrococcus (2013), Biochemistry, 52, 2764-2773.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.14 expressed in Escherichia coli BL21(DE3)pLysS cells Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.14 hanging drop and sitting drop vapor diffusion methods, using 100 mM Tris, pH 8.0, 2-3 M 1,6-hexanediol, and 200 mM MgCl2 Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.14 0.009
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii
1.8.1.14 0.073
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.14 CoA-disulfide + NADH + H+ Pyrococcus horikoshii
-
 CoA + NAD+
-
 ?
1.8.1.14 CoA-disulfide + NADPH + H+ Pyrococcus horikoshii
-
 CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.14 Pyrococcus horikoshii O58308
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.14 Ni-NTA column chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.14 CoA-disulfide + NADH + H+
-
 Pyrococcus horikoshii CoA + NAD+
-
 ?
1.8.1.14 CoA-disulfide + NADPH + H+
-
 Pyrococcus horikoshii CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.14 CoADR
-
 Pyrococcus horikoshii
1.8.1.14 coenzyme A disulfide reductase
-
 Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.14 7.2
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii
1.8.1.14 8.1
-
 NADPH at 50°C and pH 8.1 in 1 M Tris buffer Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.14 coenzyme A
-
 Pyrococcus horikoshii
1.8.1.14 FAD
-
 Pyrococcus horikoshii
1.8.1.14 NADH
-
 Pyrococcus horikoshii
1.8.1.14 NADPH the enzyme shows a preference for NADPH (10fold lower Km), although it makes efficient use of NADH as well Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
1.8.1.14 physiological function the enzyme is not essential for S0 respiration in Pyrococcus but appears to participate in oxidative defense in the presence of elemental sulfur Pyrococcus horikoshii