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Literature summary extracted from

  • Nielsen, J.W.; Poulsen, N.R.; Johnsson, A.; Winther, J.R.; Stipp, S.L.; Willemoes, M.
    Metal-ion dependent catalytic properties of Sulfolobus solfataricus class ii alpha-mannosidase (2012), Biochemistry, 51, 8039-8046.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.24
-
 Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Cu2+ inhibits in presence of Co2+ Saccharolobus solfataricus
3.2.1.24 Ni2+ inhibits in presence of Co2+ Saccharolobus solfataricus
3.2.1.24 swainsonine the enzyme substituted with Zn2+ is considerably less sensitive to swainsonine compared with the Co2+-, Cd2+-, and Mn2+-substituted enzyme Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.11
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 0.39
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 0.65
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 0.85
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 0.99
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
3.2.1.24 1
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
3.2.1.24 1.16
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 2
-
 alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 2
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 2.3
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 2.5
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Cd2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
3.2.1.24 Co2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
3.2.1.24 Mn2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
3.2.1.24 Zn2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Saccharolobus solfataricus Q97UK5
-
-
3.2.1.24 Saccharolobus solfataricus P2 Q97UK5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 4-nitrophenyl alpha-D-mannoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.24 4-nitrophenyl alpha-D-mannoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->2)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->2)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->3)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->3)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->4)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->4)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->6)-D-Man + H2O
-
 Saccharolobus solfataricus 2 alpha-D-mannose
-
 ?
3.2.1.24 alpha-D-Man-(1->6)-D-Man + H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-mannose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 GH38 class II alpha-mannosidase
-
 Saccharolobus solfataricus
3.2.1.24 ManA
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.24 1.9
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 2
-
 alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 3.1
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 4.8
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 4.9
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 5.5
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 10.4
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 10.5
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
3.2.1.24 12.5
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 13.6
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 16.1
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.24 0.102
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 0.106
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 0.185
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 0.206
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 0.277
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
3.2.1.24 0.28
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
3.2.1.24 0.31
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
3.2.1.24 0.95
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 1
-
 alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 1.3
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 4.2
-
 alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 4.2
-
 alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 6.4
-
 alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.2.1.24 10.5
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
3.2.1.24 12
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
3.2.1.24 16
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
3.2.1.24 21
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
3.2.1.24 120
-
 4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus