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Literature summary extracted from

  • Makowska-Grzyska, M.; Kim, Y.; Wu, R.; Wilton, R.; Gollapalli, D.R.; Wang, X.K.; Zhang, R.; Jedrzejczak, R.; Mack, J.C.; Maltseva, N.; Mulligan, R.; Binkowski, T.A.; Gornicki, P.; Kuhn, M.L.; Anderson, W.F.; Hedstrom, L.; Joachimiak, A.
    Bacillus anthracis inosine 5-monophosphate dehydrogenase in action: the first bacterial series of structures of phosphate ion-, substrate-, and product-bound complexes (2012), Biochemistry, 51, 6148-6163.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.205 Cs+ 36% of the activity with K+ Bacillus anthracis
1.1.1.205 dimethyl sulfoxide up to 15-20% increase IMP dehydrogenase activity Bacillus anthracis
1.1.1.205 K+ optimal activation at 100-150 mM K+ Bacillus anthracis
1.1.1.205 Li+ less than 1% of the activity with K+ Bacillus anthracis
1.1.1.205 additional information strict requirement for a monovalent cation Bacillus anthracis
1.1.1.205 Na+ less than 1% of the activity with K+ Bacillus anthracis
1.1.1.205 NH4+ 77% of the activity with K+ Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.205 in a phosphate ion-bound form and in complex with its substrate, inosine 5'-monophosphate, and product, xanthosine 5'-monophosphate, to 2.38-2.65 A resolution. The enzyme monomer has a typical two-domain structure, the catalytic domain, which is a TIM barrel, and the CBS domain. In all structures, each monomer contains a ligand bound in the active site, i.e.phosphate anion in the apo structure and IMP and XMP in the substrate and product-bound structures, respectively. In all the structures, the CBS domains are partially disordered Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide
-
 Bacillus anthracis
1.1.1.205 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide
-
 Campylobacter jejuni
1.1.1.205 Mycophenolic acid noncompetitive with regard to inosine monophosphate and NAD+ Bacillus anthracis
1.1.1.205 N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Bacillus anthracis
1.1.1.205 N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
-
 Campylobacter jejuni
1.1.1.205 NAD+
-
 Bacillus anthracis
1.1.1.205 xanthosine 5'-phosphate competitive with regard to inosine monophosphate Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.018
-
 Inosine 5'-diphosphate pH 8.0, 22°C Bacillus anthracis
1.1.1.205 0.55
-
 NAD+ pH 8.0, 22°C Bacillus anthracis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.205 dimethyl sulfoxide up to 15-20% increase IMP dehydrogenase activity Campylobacter jejuni
1.1.1.205 dimethyl sulfoxide up to 15-20% increase IMP dehydrogenase activity Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Bacillus anthracis Q81W29
-
-
1.1.1.205 Campylobacter jejuni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 inosine 5'-diphosphate + NAD+ + H2O
-
 Campylobacter jejuni xanthosine 5'-diphosphate + NADH + H+
-
 ?
1.1.1.205 inosine 5'-diphosphate + NAD+ + H2O
-
 Bacillus anthracis xanthosine 5'-diphosphate + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.205 1.4
-
 Inosine 5'-diphosphate pH 8.0, 22°C Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.205 8.2
-
-
 Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+
-
 Campylobacter jejuni
1.1.1.205 NAD+
-
 Bacillus anthracis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.205 0.0039
-
 Mycophenolic acid substrate NAD+, pH 8.0, 22°C Bacillus anthracis
1.1.1.205 0.0048
-
 Mycophenolic acid substrate inosine monophosphate, pH 8.0, 22°C Bacillus anthracis
1.1.1.205 0.218
-
 xanthosine 5'-phosphate pH 8.0, 22°C Bacillus anthracis
1.1.1.205 3.9
-
 NAD+ pH 8.0, 22°C Bacillus anthracis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.205 0.000051
-
 pH 8.0, 22°C Campylobacter jejuni N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
1.1.1.205 0.000057
-
 pH 8.0, 22°C Bacillus anthracis 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide
1.1.1.205 0.000057
-
 pH 8.0, 22°C Bacillus anthracis N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide
1.1.1.205 0.00012
-
 pH 8.0, 22°C Campylobacter jejuni 4-[(1R)-1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl]ethoxy]quinoline 1-oxide