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Literature summary extracted from

  • Orban, T.; Huang, C.C.; Homan, K.T.; Jastrzebska, B.; Tesmer, J.J.; Palczewski, K.
    Substrate-induced changes in the dynamics of rhodopsin kinase (G protein-coupled receptor kinase 1) (2012), Biochemistry, 51, 3404-3411.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 expressed in Escherichia coli as a His-tagged fusion protein Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.14 DELTA20-535 deletion of 19 N-terminal amino acids results in increased flexibility in the active site and interdomain contacts of this enzyme: peptides that directly interact with ATP are not as highly stabilized by adding Mg2+-ATP, and dynamics are greater in the interface between the large lobe of the kinase domain and the regulator of the G protein signaling homology domain compared to wild-type Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect ADP + phosphorhodopsin
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?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 using Ni-NTA chromatography Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect Bos taurus ADP + phosphorhodopsin
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?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.14 GRK1
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Bos taurus