Literature summary extracted from
Orban, T.; Huang, C.C.; Homan, K.T.; Jastrzebska, B.; Tesmer, J.J.; Palczewski, K.
Substrate-induced changes in the dynamics of rhodopsin kinase (G protein-coupled receptor kinase 1) (2012), Biochemistry, 51, 3404-3411.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.11.14 |
expressed in Escherichia coli as a His-tagged fusion protein |
Bos taurus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.11.14 |
DELTA20-535 |
deletion of 19 N-terminal amino acids results in increased flexibility in the active site and interdomain contacts of this enzyme: peptides that directly interact with ATP are not as highly stabilized by adding Mg2+-ATP, and dynamics are greater in the interface between the large lobe of the kinase domain and the regulator of the G protein signaling homology domain compared to wild-type |
Bos taurus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.11.14 |
ATP + rhodopsin |
Bos taurus |
conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect |
ADP + phosphorhodopsin |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.14 |
Bos taurus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.14 |
using Ni-NTA chromatography |
Bos taurus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.11.14 |
ATP + rhodopsin |
conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect |
Bos taurus |
ADP + phosphorhodopsin |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.14 |
GRK1 |
- |
Bos taurus |