EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.21 | expressed in Escherichia coli DH5alpha cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.3.21 | hanging drop vapor diffusion method, using 0.7 M potassium/sodium tartrate, 100 mM imidazole (pH 7.4), and 200 mM NaCl, at room temperature | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.21 | L469G | the mutant shows increased affinity for benzylamine and methylamine compared to the wild type enzyme | Homo sapiens |
1.4.3.21 | M211V | the mutant shows increased affinity for benzylamine and methylamine compared to the wild type enzyme | Homo sapiens |
1.4.3.21 | M211V/Y394N/L469G | the mutant of AOC3 changes substrate preferences toward those of copper-containing monoamine oxidase AOC2 with high activity towards 2-phenylethylamine | Homo sapiens |
1.4.3.21 | T212A | the mutant shows increased affinity for benzylamine and reduced affinity for methylamine compared to the wild type enzyme | Homo sapiens |
1.4.3.21 | Y394N | the mutant shows reduced affinity for benzylamine and methylamine compared to the wild type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.21 | imidazole | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.21 | 0.013 | - |
benzylamine | mutant enzyme L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.018 | - |
benzylamine | mutant enzyme M211V/Y394N/L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.025 | - |
benzylamine | mutant enzyme M211V, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.084 | - |
benzylamine | mutant enzyme T212A, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.184 | - |
benzylamine | wild type enzyme, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.215 | - |
benzylamine | mutant enzyme Y394N, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.236 | - |
methylamine | mutant enzyme L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.28 | - |
methylamine | mutant enzyme M211V, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.331 | - |
methylamine | wild type enzyme, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 0.502 | - |
methylamine | mutant enzyme T212A, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 1.26 | - |
methylamine | mutant enzyme Y394N, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens | |
1.4.3.21 | 2.043 | - |
methylamine | mutant enzyme M211V/Y394N/L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.21 | Homo sapiens | Q16853 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.21 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.21 | blood plasma | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.21 | 2-phenylethylamine + H2O + O2 | - |
Homo sapiens | 2-phenylethanal + NH3 + H2O2 | - |
? | |
1.4.3.21 | 4-tyramine + H2O + O2 | - |
Homo sapiens | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | benzylamine + H2O + O2 | - |
Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | methylamine + H2O + O2 | - |
Homo sapiens | formaldehyde + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.21 | AOC3 | - |
Homo sapiens |
1.4.3.21 | copper-containing monoamine oxidase | - |
Homo sapiens |
1.4.3.21 | primary amine oxidase | - |
Homo sapiens |
1.4.3.21 | VAP-1 | - |
Homo sapiens |
1.4.3.21 | vascular adhesion protein-1 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.21 | 2,4,5-trihydroxyphenylalanine quinone | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.3.21 | physiological function | the enzymatic activity plays a crucial role in leukocyte trafficking | Homo sapiens |