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Literature summary extracted from

  • Elovaara, H.; Kidron, H.; Parkash, V.; Nymalm, Y.; Bligt, E.; Ollikka, P.; Smith, D.; Pihlavisto, M.; Salmi, M.; Jalkanen, S.; Salminen, T.
    Identification of two imidazole binding sites and key residues for substrate specificity in human primary amine oxidase AOC3 (2011), Biochemistry, 50, 5507-5520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 expressed in Escherichia coli DH5alpha cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.21 hanging drop vapor diffusion method, using 0.7 M potassium/sodium tartrate, 100 mM imidazole (pH 7.4), and 200 mM NaCl, at room temperature Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.21 L469G the mutant shows increased affinity for benzylamine and methylamine compared to the wild type enzyme Homo sapiens
1.4.3.21 M211V the mutant shows increased affinity for benzylamine and methylamine compared to the wild type enzyme Homo sapiens
1.4.3.21 M211V/Y394N/L469G the mutant of AOC3 changes substrate preferences toward those of copper-containing monoamine oxidase AOC2 with high activity towards 2-phenylethylamine Homo sapiens
1.4.3.21 T212A the mutant shows increased affinity for benzylamine and reduced affinity for methylamine compared to the wild type enzyme Homo sapiens
1.4.3.21 Y394N the mutant shows reduced affinity for benzylamine and methylamine compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 imidazole
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.013
-
benzylamine mutant enzyme L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.018
-
benzylamine mutant enzyme M211V/Y394N/L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.025
-
benzylamine mutant enzyme M211V, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.084
-
benzylamine mutant enzyme T212A, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.184
-
benzylamine wild type enzyme, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.215
-
benzylamine mutant enzyme Y394N, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.236
-
methylamine mutant enzyme L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.28
-
methylamine mutant enzyme M211V, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.331
-
methylamine wild type enzyme, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 0.502
-
methylamine mutant enzyme T212A, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 1.26
-
methylamine mutant enzyme Y394N, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens
1.4.3.21 2.043
-
methylamine mutant enzyme M211V/Y394N/L469G, in Krebs Ringer phosphate glucose buffer, pH 7.4, at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Homo sapiens Q16853
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.21 blood plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 2-phenylethylamine + H2O + O2
-
Homo sapiens 2-phenylethanal + NH3 + H2O2
-
?
1.4.3.21 4-tyramine + H2O + O2
-
Homo sapiens (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 benzylamine + H2O + O2
-
Homo sapiens benzaldehyde + NH3 + H2O2
-
?
1.4.3.21 methylamine + H2O + O2
-
Homo sapiens formaldehyde + NH3 + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 AOC3
-
Homo sapiens
1.4.3.21 copper-containing monoamine oxidase
-
Homo sapiens
1.4.3.21 primary amine oxidase
-
Homo sapiens
1.4.3.21 VAP-1
-
Homo sapiens
1.4.3.21 vascular adhesion protein-1
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
Homo sapiens

General Information

EC Number General Information Comment Organism
1.4.3.21 physiological function the enzymatic activity plays a crucial role in leukocyte trafficking Homo sapiens