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Literature summary extracted from

  • Luka, Z.; Moss, F.; Loukachevitch, L.V.; Bornhop, D.J.; Wagner, C.
    Histone demethylase LSD1 is a folate-binding protein (2011), Biochemistry, 50, 4750-4756.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.66 additional information binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, overview Homo sapiens
1.14.99.66 tetrahydrofolate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.66 nucleus LSD1 is associated with folate Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.66 95059
-
 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry Homo sapiens
1.14.99.66 100000
-
 about, recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.66 histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 additional information Homo sapiens LSD1 removes the methyl groups from lysines 4 and 9 of histone 3 with the generation of formaldehyde from the methyl group ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.66 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.66 recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.66 HeLa cell LSD1 is associated with folate Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.66 histone H3 N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 additional information LSD1 removes the methyl groups from lysines 4 and 9 of histone 3 with the generation of formaldehyde from the methyl group Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.66 monomer 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.99.66 histone demethylase
-
 Homo sapiens
1.14.99.66 LSD1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.66 22
-
 assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.66 7.2 7.5 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.66 FAD a flavin-containing enzyme Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.99.66 additional information mechanism of histone H3 demethylation by demethylase LSD1, overview Homo sapiens
1.14.99.66 physiological function methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demethylases Homo sapiens