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Literature summary extracted from
- The activity of barley NADPH-dependent thioredoxin reductase C is independent of the oligomeric state of the protein: tetrameric structure determined by cryo-electron microscopy (2011), Biochemistry, 50, 3713-3723.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | expressed in Escherichia coli BL21 pLysS cells | Hordeum vulgare |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.1.9 | etioplast | - |
Hordeum vulgare | 9513 | - |
| 1.8.1.9 | soluble | - |
Hordeum vulgare | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 54662 | - |
4 * 54662, calculated from sequence of cDNA | Hordeum vulgare |
| 1.8.1.9 | 63000 | - |
4 * 63000, SDS-PAGE | Hordeum vulgare |
| 1.8.1.9 | 200000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin disulfide + NADPH + H+ | Hordeum vulgare | - |
thioredoxin + NADP+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Hordeum vulgare | B0FXK2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | Ni2+ affinity column chromatography and Superose 6 gel filtration | Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoicacid) + NADPH + H+ | - |
Hordeum vulgare | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
| 1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Hordeum vulgare | thioredoxin + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | homotetramer | 4 * 63000, SDS-PAGE | Hordeum vulgare |
| 1.8.1.9 | homotetramer | 4 * 54662, calculated from sequence of cDNA | Hordeum vulgare |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | NADPH-dependent thioredoxin reductase C | - |
Hordeum vulgare |
| 1.8.1.9 | NTRC | - |
Hordeum vulgare |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Hordeum vulgare | |
| 1.8.1.9 | NADPH | - |
Hordeum vulgare | |
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Release 2023.1 (January 2023)
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