EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.1.1.2 | additional information | construction of NuoL variants Y544Stop and W592Stop, that contain a stop codon leading to truncations in themiddle and at the end of the horizontal helix. The W592Stop variant is missing the C-terminal TM helix and the Y544Stop variant is missing this helix and approximately half of the amphipathic helix. Proton translocation by the mutant W592Stop variant and the Y544Stop variant after reconstitution in proteoliposomes, overview | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.1.1.2 | membrane | - |
Escherichia coli | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | Escherichia coli | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | Escherichia coli BW25113 | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.1.2 | Escherichia coli | - |
mutant derivative of strain BW25113 from which the nuo operon is deleted by genomic replacement | - |
7.1.1.2 | Escherichia coli BW25113 | - |
mutant derivative of strain BW25113 from which the nuo operon is deleted by genomic replacement | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | - |
Escherichia coli | NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | - |
Escherichia coli BW25113 | NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.1.2 | NADH:ubiquinone oxidoreductase | - |
Escherichia coli |
7.1.1.2 | respiratory complex I | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.1.1.2 | 30 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.1.1.2 | 6 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.2 | NADH | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.1.1.2 | malfunction | electron transfer and proton translocation activity of complex I variants lacking either NuoL or parts of the C-terminal domain, overview | Escherichia coli |
7.1.1.2 | additional information | the complex consists of a peripheral arm catalyzing the electron transfer reaction and a membrane arm involved in proton translocation. The energy released by the redox reaction is transmitted to the membrane arm via a conformational change in the horizontal helix. The helix corresponds to the C-terminal part of the most distal subunit NuoL. The C-terminal domain of NuoL is essential for the translocation of 2H+/2e- | Escherichia coli |
7.1.1.2 | physiological function | respiratory complex I couples the transfer of electrons from NADH to ubiquinone with a translocation of protons across the membrane | Escherichia coli |