EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.42 | expressed in Escherichia coli strain TB1 | Marinobacter nauticus |
1.2.1.50 | plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression | Marinobacter nauticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.42 | 0.004 | - |
hexadecanoyl-CoA | at pH 7.0 and 37°C | Marinobacter nauticus | |
1.2.1.42 | 0.05 | - |
cis-11-hexadecenal | at pH 7.0 and 37°C | Marinobacter nauticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.42 | 116000 | - |
x * 116000, SDS-PAGE | Marinobacter nauticus |
1.2.1.50 | 116000 | - |
SDS-PAGE, in agreement with mass calculated from amino acid sequence | Marinobacter nauticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.42 | arachidonoyl-CoA + NADPH + H+ | Marinobacter nauticus | 86% activity compared to hexadecanoyl-CoA | arachidonic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | cis-11-hexadecenal + CoA + NADP+ | Marinobacter nauticus | - |
(E)-hexadec-11-enoyl-CoA + NADPh + H+ | - |
? | |
1.2.1.42 | dodecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 26% activity compared to hexadecanoyl-CoA | dodecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | hexadecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 100% activity | 1-hexadecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | additional information | Marinobacter nauticus | the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal | ? | - |
? | |
1.2.1.42 | octadecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 69% activity compared to hexadecanoyl-CoA | octadecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | octanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 9% activity compared to hexadecanoyl-CoA | octanal + CoA + NADP+ | - |
? | |
1.2.1.42 | oleoyl-CoA + NADPH + H+ | Marinobacter nauticus | 78% activity compared to hexadecanoyl-CoA | oleic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | palmitoleoyl-CoA + NADPH + H+ | Marinobacter nauticus | 99% activity compared to hexadecanoyl-CoA | palmitoleic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | tetradecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 60% activity compared to hexadecanoyl-CoA | tetradecanal + CoA + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.42 | Marinobacter nauticus | - |
- |
- |
1.2.1.50 | Marinobacter nauticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.42 | amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration | Marinobacter nauticus |
1.2.1.50 | by amylose affinity chromatography and metal affinity chromatography with nickel | Marinobacter nauticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.42 | 0.015 | - |
using octanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.034 | - |
using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.04 | - |
using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.044 | - |
using oleoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.049 | - |
using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.057 | - |
using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
1.2.1.42 | 0.057 | - |
using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.42 | arachidonoyl-CoA + NADPH + H+ | 86% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | arachidonic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | cis-11-hexadecenal + CoA + NADP+ | - |
Marinobacter nauticus | (E)-hexadec-11-enoyl-CoA + NADPh + H+ | - |
? | |
1.2.1.42 | dodecanoyl-CoA + NADPH + H+ | 26% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | dodecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | hexadecanoyl-CoA + NADPH + H+ | 100% activity | Marinobacter nauticus | 1-hexadecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | additional information | the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal | Marinobacter nauticus | ? | - |
? | |
1.2.1.42 | octadecanoyl-CoA + NADPH + H+ | 69% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | octadecanal + CoA + NADP+ | - |
? | |
1.2.1.42 | octanoyl-CoA + NADPH + H+ | 9% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | octanal + CoA + NADP+ | - |
? | |
1.2.1.42 | oleoyl-CoA + NADPH + H+ | 78% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | oleic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | palmitoleoyl-CoA + NADPH + H+ | 99% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | palmitoleic aldehyde + CoA + NADP+ | - |
? | |
1.2.1.42 | tetradecanoyl-CoA + NADPH + H+ | 60% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | tetradecanal + CoA + NADP+ | - |
? | |
1.2.1.50 | arachidonoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal + CoA + NADP+ | further reduction of (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal into the corresponding (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraen-1-ol | ? | |
1.2.1.50 | fatty acyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | fatty aldehyde + CoA + NADP+ | reaction intermediate, enzyme converts fatty acyl-CoA directly into the corresponding fatty alcohol by four-electron reduction, free fatty aldehyde intermediate is not accessible | ? | |
1.2.1.50 | lauroyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | dodecanal + CoA + NADP+ | further reduction of dodecanal into the corresponding dodecan-1-ol | ? | |
1.2.1.50 | additional information | no reaction product detected with NADH or free fatty acids | Marinobacter nauticus | ? | - |
? | |
1.2.1.50 | myristoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | tetradecanal + CoA + NADP+ | further reduction of tetradecanal into the corresponding tetradecan-1-ol | ? | |
1.2.1.50 | octanoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | octanal + NADP+ | further reduction of octanal into the corresponding octan-1-ol | ? | |
1.2.1.50 | oleoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (9Z)-octadec-9-enal + CoA + NADP+ | further reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol | ? | |
1.2.1.50 | palmitoleoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (9Z)-hexadec-9-enal + CoA + NADP+ | further reduction of (9Z)-hexadec-9-enal into the corresponding (9Z)-hexadec-9-en-1-ol | ? | |
1.2.1.50 | palmitoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | hexadecanal + CoA + NADP+ | further reduction of hexadecanal into the corresponding hexadecan-1-ol | ? | |
1.2.1.50 | stearoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | octadecanal + CoA + NADP+ | further reduction of octadecanal into the corresponding octadecan-1-ol | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.42 | ? | x * 116000, SDS-PAGE | Marinobacter nauticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.42 | FACoAR | - |
Marinobacter nauticus |
1.2.1.42 | fatty acyl-CoA reductase | - |
Marinobacter nauticus |
1.2.1.50 | FACoAR | - |
Marinobacter nauticus |
1.2.1.50 | fatty acyl-CoA reductase | - |
Marinobacter nauticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.42 | 7 | - |
- |
Marinobacter nauticus |
1.2.1.50 | 7 | - |
- |
Marinobacter nauticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.42 | 6.5 | 9 | optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0 | Marinobacter nauticus |
1.2.1.50 | 6.5 | 9 | activity falling dramatically below pH 6.5 and above 9.0 | Marinobacter nauticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.42 | NADPH | - |
Marinobacter nauticus |