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Literature summary extracted from

  • Farrar, C.E.; Siu, K.K.; Howell, P.L.; Jarrett, J.T.
    Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of inhibition by products and product-related biomolecules (2010), Biochemistry, 49, 9985-9996.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6 expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.6 5'-deoxyadenosine product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. Escherichia coli
2.8.1.6 L-methionine product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme. Escherichia coli
2.8.1.6 additional information 5'-methylthioadenosine is not an inhibitor. Neither biotin nor iminobiotin is a significant inhibitor Escherichia coli
2.8.1.6 S-adenosyl-L-homocysteine potent inhibitor Escherichia coli
2.8.1.6 sinefungin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.6 0.01
-
 S-adenosyl-L-methionine in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + [S] + S-adenosyl-L-methionine
-
 Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 dimer
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.1.6 0.00065
-
 S-adenosyl-L-homocysteine in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli
2.8.1.6 0.075
-
 sinefungin in 50 mM Tris-HCl, 100 mM KCl, and 5 mM dithiothreitol, pH 8.0, at 37°C Escherichia coli