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Literature summary extracted from

  • Dey, M.; Li, X.; Kunz, R.C.; Ragsdale, S.W.
    Detection of organometallic and radical intermediates in the catalytic mechanism of methyl-coenzyme M reductase using the natural substrate methyl-coenzyme M and a coenzyme B substrate analogue (2010), Biochemistry, 49, 10902-10911.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 0.03
-
 CH3-S-CoM with SH-CoB6 as cosubstrate, pH not specified in the publication, at 25°C Methanothermobacter marburgensis
2.8.4.1 0.08
-
 SH-CoB6 pH not specified in the publication, at 25°C Methanothermobacter marburgensis
2.8.4.1 0.28
-
 CH3-S-CoM with SH-CoB5 as cosubstrate, pH not specified in the publication, at 25°C Methanothermobacter marburgensis
2.8.4.1 0.34
-
 SH-CoB5 pH not specified in the publication, at 25°C Methanothermobacter marburgensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Nickel the active site of the enzyme contains an essential redox-active nickel tetrapyrrole cofactor, coenzyme F430, which is active in the Ni(I) state Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 CH3-S-CoM + SH-CoB Methanothermobacter marburgensis
-
 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB Methanothermobacter marburgensis OCM82
-
 CoM-S-S-CoB + methane
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter marburgensis
-
-
-
2.8.4.1 Methanothermobacter marburgensis OCM82
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 CH3-S-CoM + SH-CoB
-
 Methanothermobacter marburgensis CoM-S-S-CoB + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB
-
 Methanothermobacter marburgensis OCM82 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB5
-
 Methanothermobacter marburgensis CoM-S-S-CoB5 + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB5
-
 Methanothermobacter marburgensis OCM82 CoM-S-S-CoB5 + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB6 methanogenesis occurs 1000fold more slowly than with SH-CoB Methanothermobacter marburgensis CoM-S-S-CoB6 + methane
-
 ?
2.8.4.1 CH3-S-CoM + SH-CoB6 methanogenesis occurs 1000fold more slowly than with SH-CoB Methanothermobacter marburgensis OCM82 CoM-S-S-CoB6 + methane
-
 ?
2.8.4.1 additional information no activity with SH-CoB8 or SH-CoB9 Methanothermobacter marburgensis ?
-
 ?
2.8.4.1 additional information no activity with SH-CoB8 or SH-CoB9 Methanothermobacter marburgensis OCM82 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.4.1 MCR
-
 Methanothermobacter marburgensis
2.8.4.1 methyl-coenzyme M reductase
-
 Methanothermobacter marburgensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 the active site of the enzyme contains an essential redox-active nickel tetrapyrrole cofactor, coenzyme F430, which is active in the Ni(I) state Methanothermobacter marburgensis