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Literature summary extracted from

  • Reeksting, S.; Müller, I.; Burger, P.; Burgos, E.; Salmon, L.; Louw, A.; Birkholtz, L.; Wrenger, C.
    Exploring inhibition of Pdx1, a component of the PLP synthase complex of the human malaria parasite Plasmodium falciparum (2013), Biochem. J., 449, 175-187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 Strep-Tag-fusion proteins expressed in Escherichia coli BLR (DE3) Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.6 D26A/K83A/K151A no formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein) Plasmodium falciparum
4.3.3.6 E136A/R139A/R140A formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (also observed with wild type protein) Plasmodium falciparum
4.3.3.6 additional information multiple deletion mutant proteins Plasmodium falciparum
4.3.3.6 R167A reduced formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein), 50% wild type activity Plasmodium falciparum
4.3.3.6 R85A/H88A/E91A no formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein), dodecameric assembly prevented Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.6 2-deoxy-D-ribose 5-phosphate at 12 mM 56% residual activity Plasmodium falciparum
4.3.3.6 4-phospho-D-erythronhydrazide
-
 Plasmodium falciparum
4.3.3.6 D-erythronhydrazide
-
 Plasmodium falciparum
4.3.3.6 D-erythrose
-
 Plasmodium falciparum
4.3.3.6 D-erythrose 4-phosphate
-
 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6 affinity chromatography using the Strep-Tag Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
-
 Plasmodium falciparum pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 Pdx1 pyridoxal 5'-phosphate synthase complex consits of Pdx1 and Pdx2 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.3.6 0.016
-
 Pdx1 in the heteromeric pyridoxal 5'-phosphate synthase complex, pH 8.0, 37°C Plasmodium falciparum 4-phospho-D-erythronhydrazide
4.3.3.6 0.043
-
 Pdx1, pH 8.0, 37°C Plasmodium falciparum 4-phospho-D-erythronhydrazide
4.3.3.6 0.902
-
 Pdx1, pH 8.0, 37°C Plasmodium falciparum D-erythronhydrazide
4.3.3.6 3.7
-
 Pdx1, pH 8.0, 37°C Plasmodium falciparum D-erythrose 4-phosphate
4.3.3.6 160
-
 Pdx1, pH 8.0, 37°C Plasmodium falciparum D-erythrose