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Literature summary extracted from

  • Garcia-Ruiz, E.; Gonzalez-Perez, D.; Ruiz-Duenas, F.J.; Martinez, A.T.; Alcalde, M.
    Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase (2012), Biochem. J., 441, 487-498.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expression in Saccharomyces cerevisiae Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 E37K/V160A/T184M/Q202L mutant obtained by directed evolution, increase in activity and temperature stability Pleurotus eryngii
1.11.1.16 additional information an engineered N-terminally truncated variant of mutant E37K/V160A/T184M/Q202L displays similar biochemical properties to those of the non-truncated counterpart in terms of kinetics, stability and spectroscopic features. Additional cycles of evolution raised the melting temperature by 8 degrees and significantly increased the enzyme's stability at alkaline pHs. In addition, the Km for H2O2 is enhanced up to 15fold while the catalytic efficiency is maintained, and there is an improvement in peroxide stability Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.0066
-
 Reactive Black 5 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.007
-
 Reactive Black 5 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.045
-
 Mn2+ wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.051
-
 H2O2 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.056
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.12
-
 Mn2+ mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.2
-
 H2O2 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 0.54
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 6.5
-
 2,6-dimethoxyphenol mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 32
-
 2,6-dimethoxyphenol wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.16 35244
-
 x * 35258, calculated, x * 35244, MALDI-TOF Pleurotus eryngii
1.11.1.16 35258
-
 x * 35258, calculated, x * 35244, MALDI-TOF Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753 isoform Vpl2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.16 glycoprotein degree of glycosylation is 9% Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2 2,6-dimethoxyphenol + 2 H2O2
-
 Pleurotus eryngii coerulignone + 2 H2O
-
 ?
1.11.1.16 2 Mn2+ + 2 H+ + H2O2
-
 Pleurotus eryngii 2 Mn3+ + 2 H2O
-
 ?
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus eryngii ? + H2O
-
 ?
1.11.1.16 Reactive Black 5 + H2O2
-
 Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.16 ? x * 35258, calculated, x * 35244, MALDI-TOF Pleurotus eryngii

Synonyms

EC Number Synonyms Comment Organism
1.11.1.16 Vpl2
-
 Pleurotus eryngii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.16 58
-
 wild-type, melting temperature Pleurotus eryngii
1.11.1.16 59.4
-
 mutant E37K/V160A/T184M/Q202L, melting temperature Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 10.6
-
 Reactive Black 5 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 11.8
-
 Reactive Black 5 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 54
-
 Mn2+ wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 58
-
 2,6-dimethoxyphenol mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 75
-
 Mn2+ mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 98
-
 2,6-dimethoxyphenol wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 135
-
 H2O2 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 220
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 365
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 490
-
 H2O2 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.16 3
-
 wild-type Pleurotus eryngii
1.11.1.16 3.5
-
 mutant E37K/V160A/T184M/Q202L Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.16 3.1
-
 2,6-dimethoxyphenol wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 9.1
-
 2,6-dimethoxyphenol mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 410
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 630
-
 Mn2+ mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 1190
-
 Mn2+ wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 1600
-
 Reactive Black 5 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 1670
-
 Reactive Black 5 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 2400
-
 H2O2 mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 2650
-
 H2O2 wild-type, pH 3.5, temperature not specified in the publication Pleurotus eryngii
1.11.1.16 6480
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication Pleurotus eryngii