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Literature summary extracted from

  • Stiti, N.; Adewale, I.O.; Petersen, J.; Bartels, D.; Kirch, H.H.
    Engineering the nucleotide coenzyme specificity and sulfhydryl redox sensitivity of two stress-responsive aldehyde dehydrogenase isoenzymes of Arabidopsis thaliana (2011), Biochem. J., 434, 459-471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 C247S the mutant shows slightly reduced activity compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 C253S the mutation abolishes enzymatic activity Arabidopsis thaliana
1.2.1.3 C45S the mutant shows stongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 I200G the mutant also exhibits activity with NADP+ and shows decreased affinity for NAD+ compared to the wild type enzyme Arabidopsis thaliana
1.2.1.3 I200V the mutant also exhibits activity with NADP+ and shows decreased affinity for NAD+ compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.003
-
 trans-2-nonenal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.005
-
 dodecanal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.008
-
 Nonanal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.029
-
 octanal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.0403
-
 4-hydroxynonenal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.071
-
 hexanal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.119
-
 NAD+ wild type enzyme, with nonanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.18
-
 trans-2-hexenal in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.421
-
 NAD+ wild type enzyme, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.496
-
 NAD+ mutant enzyme I200V, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 0.51
-
 propionaldehyde in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana
1.2.1.3 3.218
-
 NAD+ mutant enzyme I200G, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 cytosol isoform ALDH3H1 Arabidopsis thaliana 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 56000
-
 2 * 56000, SDS-PAGE Arabidopsis thaliana
1.2.1.3 112000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Arabidopsis thaliana
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.1.3 Oxidation leads to a decrease in enzymatic activities to less than 25-35% of the activity of the corresponding reduced form. Reduction of oxidized isoform ALDH3H1 after incubation with 10 mM dithiothreitol for 1 h results in a good recovery of activity to about 83% of the initial activity, but to only 44% after reduction with 10 mM GSH Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 His-tag binding column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 (E)-2-nonenal + NAD+ + H2O
-
 Arabidopsis thaliana (E)-non-2-enoate + NADH + H+
-
 ?
1.2.1.3 4-hydroxynonenal + NAD+ + H2O
-
 Arabidopsis thaliana ? + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Arabidopsis thaliana acetate + NADH + H+
-
 ?
1.2.1.3 dodecanal + NAD+ + H2O
-
 Arabidopsis thaliana dodecanoate + NADH + H+
-
 ?
1.2.1.3 dodecanal + NAD+ + H2O best substrate Arabidopsis thaliana dodecanoate + NADH + H+
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Arabidopsis thaliana hexanoate + NADH + H+
-
 ?
1.2.1.3 additional information no activity with NADP+. Saturated aldehydes are preferred over unsaturated aldehydes, irrespective of chain length Arabidopsis thaliana ?
-
 ?
1.2.1.3 nonanal + NAD+ + H2O
-
 Arabidopsis thaliana nonanoate + NADH + H+
-
 ?
1.2.1.3 octanal + NAD+ + H2O
-
 Arabidopsis thaliana octanoate + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O
-
 Arabidopsis thaliana propanoate + NADH + H+
-
 ?
1.2.1.3 trans-2-hexenal + NAD+ + H2O
-
 Arabidopsis thaliana (E)-hex-2-enoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homodimer 2 * 56000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH3H1 cytosolic isoform Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+ dependent on, no activity with NADP+ Arabidopsis thaliana