Literature summary extracted from
Zhang, X.; Teng, Y.; Liu, J.; He, Y.; Zhou, K.; Chen, Y.; Zhou, C.
Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1 (2010), Biochem. J., 432, 445-450.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.3.6 |
His-tagged protein expressed in Escherichia coli BL21 (DE3) |
Saccharomyces cerevisiae |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.3.3.6 |
hanging-drop vapour diffusion method, 16°C |
Saccharomyces cerevisiae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.3.3.6 |
E116A |
activity as the wild-type protein |
Saccharomyces cerevisiae |
4.3.3.6 |
K117A |
no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization |
Saccharomyces cerevisiae |
4.3.3.6 |
K148A |
no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization |
Saccharomyces cerevisiae |
4.3.3.6 |
K240A |
activity as the wild-type protein |
Saccharomyces cerevisiae |
4.3.3.6 |
R136A/R137A |
no synthesis of pyridoxal 5'-phosphate, dihydroxyacetone phosphate isomerization activity as the wild-type protein |
Saccharomyces cerevisiae |
4.3.3.6 |
R164A |
completely inactive, dihydroxyacetone phosphate isomerization activity as the wild-type protein |
Saccharomyces cerevisiae |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.3.6 |
Saccharomyces cerevisiae |
Q03148 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.3.6 |
immobilized metal ion affinity chromatography (Ni2+), gel filtration |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.3.6 |
ribulose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ |
- |
Saccharomyces cerevisiae |
pyridoxal 5'-phosphate + 4 H2O + phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.3.3.6 |
pyridoxal 5-phosphate synthase Snz1 |
- |
Saccharomyces cerevisiae |
4.3.3.6 |
Snz1 |
- |
Saccharomyces cerevisiae |