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Literature summary extracted from

  • Urich, T.; Bandeiras, T.M.; Leal, S.S.; Rachel, R.; Albrecht, T.; Zimmermann, P.; Scholz, C.; Teixeira, M.; Gomes, C.M.; Kletzin, A.
    The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre (2004), Biochem. J., 381, 137-146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.55 EDTA 100 mM, slight stimulatory effect Acidianus ambivalens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.55 expression in Escherichia coli results in active, soluble SOR and in inclusion bodies from which active SOR can be refolded as long as ferric ions are present in the refolding solution. Wild-type, recombinant and refolded enzyme possesses indistinguishable properties Acidianus ambivalens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.55 Zn2+ 0.5 mM, 95% inhibition of oxygenase reaction (formation of hydrogen sulfide), 84% inhibition of reductase reaction (formation of sulfite plus thiosulfate) Acidianus ambivalens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.55 2 3 sulfur pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) Acidianus ambivalens
1.13.11.55 13
-
 sulfur pH 7.2, 65°C, reductase reaction (formation of sulphite plus thiosulfate) Acidianus ambivalens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.55 Iron the enzyme contains a low-potential mononuclear non-heme iron centre, which has a reduction potential of -268 mV at pH 6.5, one iron atom per subunit Acidianus ambivalens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.55 36000
-
 24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE Acidianus ambivalens
1.13.11.55 732000
-
 non-denaturing PAGE Acidianus ambivalens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.55 4 sulfur + 4 H2O + O2 Acidianus ambivalens initial enzyme in the sulfur-oxidation pathway 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?
1.13.11.55 4 sulfur + 4 H2O + O2 Acidianus ambivalens DSM 3772 initial enzyme in the sulfur-oxidation pathway 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.55 guanidine-HCl reversible denaturation curve with midpoint at 3.4 M guanidine-HCl Acidianus ambivalens
1.13.11.55 urea reversible denaturation curve with midpoints at 5.9 M urea Acidianus ambivalens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.55 Acidianus ambivalens P29082
-
-
1.13.11.55 Acidianus ambivalens DSM 3772 P29082
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.55
-
 Acidianus ambivalens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.13.11.55 reversible denaturation curves with midpoints at 3.4 M guanidine-HCl and 5.9 M urea Acidianus ambivalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.55 4 sulfur + 4 H2O + O2
-
 Acidianus ambivalens 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?
1.13.11.55 4 sulfur + 4 H2O + O2 initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?
1.13.11.55 4 sulfur + 4 H2O + O2
-
 Acidianus ambivalens DSM 3772 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?
1.13.11.55 4 sulfur + 4 H2O + O2 initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens DSM 3772 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
 ?
1.13.11.55 additional information not active with tetrathionate Acidianus ambivalens ?
-
 ?
1.13.11.55 additional information not active with tetrathionate Acidianus ambivalens DSM 3772 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.55 tetraicosamer 24 * 36000, ball-shaped assembly with a central hollow core probably consisting of 24 subunits in a 432 symmetry, the subunits form homodimers as the building blocks of the holoenzyme, SDS-PAGE Acidianus ambivalens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.55 SOR
-
 Acidianus ambivalens
1.13.11.55 sulphur oxygenase reductase
-
 Acidianus ambivalens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.55 0.1
-
 sulfur pH 7.2, 65°C, reductase reaction (formation of sulfite plus thiosulfate) Acidianus ambivalens
1.13.11.55 2.2
-
 sulfur pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide) Acidianus ambivalens

General Information

EC Number General Information Comment Organism
1.13.11.55 physiological function initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens