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Literature summary extracted from

  • Ohta, T.; Ishikura, S.; Shintani, S.; Usami, N.; Hara, A.
    Kinetic alteration of a human dihydrodiol/3alpha-hydroxysteroid dehydrogenase isoenzyme, AKR1C4, by replacement of histidine-216 with tyrosine or phenylalanine (2000), Biochem. J., 352, 685-691.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.357 (R)-ibuprofen 167% activity at 1 mM Homo sapiens
1.1.1.357 Clofibric acid 240% activity at 0.1 mM Homo sapiens
1.1.1.357 D-thyroxine 260% activity at 1 mM Homo sapiens
1.1.1.357 L-thyroxine 237% activity at 1 mM Homo sapiens
1.1.1.357 Sulfobromophthalein 328% activity at 0.005 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.357 expressed in Escherichia coli JM109 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.357 H216F the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme Homo sapiens
1.1.1.357 H216L inactive Homo sapiens
1.1.1.357 H216W inactive Homo sapiens
1.1.1.357 H216Y the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.357 17beta-estradiol competitive inhibitor Homo sapiens
1.1.1.357 3,5,3',5'-tetraiodothyropropionic acid competitive inhibitor Homo sapiens
1.1.1.357 Flufenamic acid competitive inhibitor Homo sapiens
1.1.1.357 Phenolphthalein competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.357 0.0005
-
 NADP+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0005
-
 androsterone wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0006
-
 NADP+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0008
-
 5alpha-androstane-3alpha,17beta-diol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0009
-
 5beta-Androstan-3alpha-ol-17-one wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.001
-
 lithocholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0011
-
 chenodeoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0012
-
 5beta-androstane-3alpha,17beta-diol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0013
-
 deoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0014
-
 deoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0014
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0017
-
 NADP+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0018
-
 lithocholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0018
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0019
-
 lithocholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0021
-
 5beta-androstane-3alpha,17beta-diol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0022
-
 androsterone mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0022
-
 5beta-androstane-3alpha,17beta-diol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0023
-
 chenodeoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0026
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0027
-
 androsterone mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0029
-
 chenodeoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0031
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.0097
-
 deoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.032
-
 cholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.041
-
 cholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.059
-
 cholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.11
-
 (S)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.146
-
 (S)-indan-1-ol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.16
-
 (R)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.24
-
 (S)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.28
-
 (S)-indan-1-ol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.29
-
 (S)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.38
-
 (R)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.42
-
 (S)-indan-1-ol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.68
-
 (R)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1
-
 NAD+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.2
-
 NAD+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.2
-
 NAD+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.357 36000
-
 x * 36000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.357 (R)-tetralol + NADP+ Homo sapiens
-
 ? + NADPH + H+
-
 ?
1.1.1.357 (S)-indan-1-ol + NADP+ Homo sapiens
-
 indan-1-one + NADPH + H+
-
 ?
1.1.1.357 (S)-tetralol + NADP+ Homo sapiens
-
 1-tetralone + NADPH + H+
-
 ?
1.1.1.357 5alpha-androstane-3alpha,17beta-diol + NADP+ Homo sapiens
-
 (5alpha)-androstan-17-beta-ol-3-one + NADPH + H+
-
 ?
1.1.1.357 5beta-androstan-3alpha-ol-17-one + NADP+ Homo sapiens
-
 (5beta)-androstan-3,17-dione + NADPH + H+
-
 ?
1.1.1.357 5beta-androstane-3alpha,17beta-diol + NADP+ Homo sapiens
-
 5beta-androstan-17beta-ol-3-one + NADPH + H+
-
 ?
1.1.1.357 androsterone + NAD+ Homo sapiens
-
 (5alpha)-androstane-3,17-dione + NADH + H+
-
 ?
1.1.1.357 androsterone + NADP+ Homo sapiens
-
 (5alpha)-androstane-3,17-dione + NADPH + H+
-
 ?
1.1.1.357 chenodeoxycholic acid + NADP+ Homo sapiens
-
 ? + NADPH + H+
-
 ?
1.1.1.357 cholic acid + NADP+ Homo sapiens
-
 ? + NADPH + H+
-
 ?
1.1.1.357 deoxycholic acid + NADP+ Homo sapiens
-
 ? + NADPH + H+
-
 ?
1.1.1.357 lithocholic acid + NADP+ Homo sapiens
-
 ? + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.357 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.357 Q-Sepharose column chromatography and Sephadex G-100 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.357 (R)-tetralol + NADP+
-
 Homo sapiens ? + NADPH + H+
-
 ?
1.1.1.357 (S)-indan-1-ol + NADP+
-
 Homo sapiens indan-1-one + NADPH + H+
-
 ?
1.1.1.357 (S)-tetralol + NADP+
-
 Homo sapiens 1-tetralone + NADPH + H+
-
 ?
1.1.1.357 5alpha-androstane-3alpha,17beta-diol + NADP+
-
 Homo sapiens (5alpha)-androstan-17-beta-ol-3-one + NADPH + H+
-
 ?
1.1.1.357 5beta-androstan-3alpha-ol-17-one + NADP+
-
 Homo sapiens (5beta)-androstan-3,17-dione + NADPH + H+
-
 ?
1.1.1.357 5beta-androstane-3alpha,17beta-diol + NADP+
-
 Homo sapiens 5beta-androstan-17beta-ol-3-one + NADPH + H+
-
 ?
1.1.1.357 androsterone + NAD+
-
 Homo sapiens (5alpha)-androstane-3,17-dione + NADH + H+
-
 ?
1.1.1.357 androsterone + NADP+
-
 Homo sapiens (5alpha)-androstane-3,17-dione + NADPH + H+
-
 ?
1.1.1.357 chenodeoxycholic acid + NADP+
-
 Homo sapiens ? + NADPH + H+
-
 ?
1.1.1.357 cholic acid + NADP+
-
 Homo sapiens ? + NADPH + H+
-
 ?
1.1.1.357 deoxycholic acid + NADP+
-
 Homo sapiens ? + NADPH + H+
-
 ?
1.1.1.357 lithocholic acid + NADP+
-
 Homo sapiens ? + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.357 ? x * 36000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.357 AKR1C4 isoform Homo sapiens
1.1.1.357 dihydrodiol/3alpha-hydroxysteroid dehydrogenase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.357 0.012
-
 deoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.018
-
 deoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.03
-
 cholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.032
-
 chenodeoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.032
-
 lithocholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.04
-
 lithocholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.04
-
 NADP+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.043
-
 lithocholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.043
-
 androsterone wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.047
-
 5beta-androstane-3alpha,17beta-diol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.048
-
 5beta-Androstan-3alpha-ol-17-one wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.05
-
 cholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.085
-
 deoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.087
-
 5alpha-androstane-3alpha,17beta-diol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.097
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.1
-
 (S)-indan-1-ol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.105
-
 chenodeoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.13
-
 chenodeoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.135
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.15
-
 androsterone mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.16
-
 NADP+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.18
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.18
-
 5beta-androstane-3alpha,17beta-diol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.18
-
 (R)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.2
-
 NAD+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.2
-
 cholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.2
-
 (S)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.25
-
 NADP+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.27
-
 (S)-indan-1-ol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.27
-
 (S)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.28
-
 androsterone mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.3
-
 5beta-androstane-3alpha,17beta-diol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.33
-
 NAD+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.35
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.38
-
 (R)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.4
-
 (S)-indan-1-ol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.43
-
 (S)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.58
-
 NAD+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 0.77
-
 (R)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.357 NAD+
-
 Homo sapiens
1.1.1.357 NADP+
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.357 0.68
-
 (S)-indan-1-ol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1
-
 cholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1
-
 (S)-indan-1-ol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1
-
 (R)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1
-
 (S)-indan-1-ol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.17
-
 (R)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.2
-
 (S)-tetralol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.2
-
 cholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.2
-
 (R)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.5
-
 (S)-tetralol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 1.83
-
 (S)-tetralol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 3.3
-
 cholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 8.8
-
 deoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 9
-
 deoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 10.3
-
 5alpha-androstane-3alpha,17beta-diol wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 14.2
-
 deoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 21.7
-
 lithocholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 23.3
-
 lithocholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 23.3
-
 NADP+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 28.3
-
 chenodeoxycholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 31.7
-
 lithocholic acid wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 45
-
 chenodeoxycholic acid mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 46.7
-
 chenodeoxycholic acid mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 53.3
-
 5beta-Androstan-3alpha-ol-17-one wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 70
-
 androsterone mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 86.7
-
 androsterone wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 96.7
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 96.7
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 101.7
-
 5beta-Androstan-3alpha-ol-17-one mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 105
-
 androsterone mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 135
-
 5alpha-androstane-3alpha,17beta-diol mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 166.7
-
 NAD+ wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 266.7
-
 NADP+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 283.3
-
 NAD+ mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 500
-
 NADP+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens
1.1.1.357 583.3
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 NAD+ mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C Homo sapiens