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Properties of catechol 2,3-dioxygenase from crude extract of Stenotrophomonas maltophilia strain KB2 immobilized in calcium alginate hydrogels

Wojcieszynska, D.; Hupert-Kocurek, K.; Jankowska, A.; Guzik, U.; Biochem. Eng. J. 66, 1-7 (2012)
No PubMed abstract available

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.2
additional information
immobilization of catechol 2,3-dioxygenase from KB2 strain using calcium alginate, the iimmobilized enzyme shows relatively higher activity against 3-methylcatechol, 4-methylcatechol, 4,5-dichlorocatechol, 3,5-dichlorocatechol, hydroquinone and tetrachlorohydroquinone than soluble enzyme, immobilization protects the enzyme from the inhibition and enhanced its resistance to inactivation during catalysis
Stenotrophomonas maltophilia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.2
CuSO4
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
1.13.11.2
H2O2
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
1.13.11.2
NaN3
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.2
0.0025
-
4-methylcatechol
pH 8.0, 35C, immobilized enzyme
Stenotrophomonas maltophilia
1.13.11.2
0.0425
-
4-methylcatechol
pH 8.0, 35C, free enzyme
Stenotrophomonas maltophilia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.2
Stenotrophomonas maltophilia
-
-
-
1.13.11.2
Stenotrophomonas maltophilia KB2
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.13.11.2
355.8
-
substrate 4-methylcatechol, pH 8.0, 35C, free enzyme
Stenotrophomonas maltophilia
1.13.11.2
936.3
-
substrate 4-methylcatechol, pH 8.0, 35C, immobilized enzyme
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.2
2,4-dichlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
2,4-dichlorophenol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
2-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
2-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
2-methylphenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3,5-dichlorocatechol + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3,5-dichlorocatechol + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
3-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
3-methylphenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4,5-dichlorocatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
butanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
catechol + O2
-
724201
Stenotrophomonas maltophilia
2-hydroxymuconate-6-semialdehyde
-
-
-
?
1.13.11.2
ethanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
hydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
hydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
methanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
propanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
tetrachlorohydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.2
35
-
assay at
Stenotrophomonas maltophilia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.2
8
-
both free and the immobilized enzyme
Stenotrophomonas maltophilia
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.2
additional information
immobilization of catechol 2,3-dioxygenase from KB2 strain using calcium alginate, the iimmobilized enzyme shows relatively higher activity against 3-methylcatechol, 4-methylcatechol, 4,5-dichlorocatechol, 3,5-dichlorocatechol, hydroquinone and tetrachlorohydroquinone than soluble enzyme, immobilization protects the enzyme from the inhibition and enhanced its resistance to inactivation during catalysis
Stenotrophomonas maltophilia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.2
CuSO4
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
1.13.11.2
H2O2
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
1.13.11.2
NaN3
the immobilized enzyme is less sensitive compared to the free enzyme
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.2
0.0025
-
4-methylcatechol
pH 8.0, 35C, immobilized enzyme
Stenotrophomonas maltophilia
1.13.11.2
0.0425
-
4-methylcatechol
pH 8.0, 35C, free enzyme
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.13.11.2
355.8
-
substrate 4-methylcatechol, pH 8.0, 35C, free enzyme
Stenotrophomonas maltophilia
1.13.11.2
936.3
-
substrate 4-methylcatechol, pH 8.0, 35C, immobilized enzyme
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.2
2,4-dichlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
2,4-dichlorophenol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
2-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
2-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
2-methylphenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3,5-dichlorocatechol + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3,5-dichlorocatechol + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
3-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
3-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
3-methylphenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4,5-dichlorocatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4-chlorophenol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
4-methylcatechol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
butanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
catechol + O2
-
724201
Stenotrophomonas maltophilia
2-hydroxymuconate-6-semialdehyde
-
-
-
?
1.13.11.2
ethanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
hydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
hydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia KB2
?
-
-
-
?
1.13.11.2
methanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
propanol + O2
-
724201
Stenotrophomonas maltophilia
?
-
-
-
?
1.13.11.2
tetrachlorohydroquinone + O2
immobilized enzyme, no activity with the free enzyme
724201
Stenotrophomonas maltophilia
?
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.2
35
-
assay at
Stenotrophomonas maltophilia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.2
8
-
both free and the immobilized enzyme
Stenotrophomonas maltophilia
General Information
EC Number
General Information
Commentary
Organism
1.13.11.2
additional information
there is a hydrophobic channel in the active site of catechol 2,3-dioxygenase. Through this channel catechol penetrates into the active site of the enzyme
Stenotrophomonas maltophilia
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.2
additional information
there is a hydrophobic channel in the active site of catechol 2,3-dioxygenase. Through this channel catechol penetrates into the active site of the enzyme
Stenotrophomonas maltophilia