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Literature summary extracted from

  • Ullah, A.; Souza, T.A.; Abrego, J.R.; Betzel, C.; Murakami, M.T.; Arni, R.K.
    Structural insights into selectivity and cofactor binding in snake venom L-amino acid oxidases (2012), Biochem. Biophys. Res. Commun., 421, 124-128.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.2 hanging drop vapor diffusion method, using 0.1 M sodium acetate trihydrate pH 4.6 and 25% (w/v) polyethylene glycol 1000 Bothrops jararacussu

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.2 Zn2+ the zinc ion plays a role in tetramer stabilization, but not in enzyme activity Bothrops jararacussu

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2 Bothrops jararacussu
-
 a 2-oxo carboxylate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops jararacussu
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 Mono Q column chromatography and Sephacryl S-100 gel filtration Bothrops jararacussu

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops jararacussu
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2
-
 Bothrops jararacussu a 2-oxo carboxylate + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2 about 20% activity compared to L-methionine Bothrops jararacussu 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-cysteine + H2O + O2 about 55% activity compared to L-methionine Bothrops jararacussu 2-oxo-3-thiopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-cystine + H2O + O2
-
 Bothrops jararacussu ?
-
 ?
1.4.3.2 L-glutamine + H2O + O2 about 11% activity compared to L-methionine Bothrops jararacussu 5-amino-2,5-dioxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-histidine + H2O + O2 about 19% activity compared to L-methionine Bothrops jararacussu 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-isoleucine + H2O + O2 about 68% activity compared to L-methionine Bothrops jararacussu 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2 about 97% activity compared to L-methionine Bothrops jararacussu 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2 about 19% activity compared to L-methionine Bothrops jararacussu 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2 100% activity Bothrops jararacussu 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2 about 74% activity compared to L-methionine Bothrops jararacussu 2-oxo-3-phenylpropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-serine + H2O + O2 about 12% activity compared to L-methionine Bothrops jararacussu 3-hydroxy-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-threonine + H2O + O2 about 11% activity compared to L-methionine Bothrops jararacussu 3-hydroxy-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-tryptophan + H2O + O2 about 63% activity compared to L-methionine Bothrops jararacussu 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-tyrosine + H2O + O2 about 68% activity compared to L-methionine Bothrops jararacussu 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-valine + H2O + O2 about 22% activity compared to L-methionine Bothrops jararacussu 3-methyl-2-oxobutanoate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 tetramer
-
 Bothrops jararacussu

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-amino acid oxidase
-
 Bothrops jararacussu
1.4.3.2 LAAO
-
 Bothrops jararacussu

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Bothrops jararacussu