BRENDA - Enzyme Database

Cellular levels of heme affect the activity of dimeric glutamyl-tRNA reductase

de Armas-Ricard, M.; Levican, G.; Katz, A.; Moser, J.; Jahn, D.; Orellana, O.; Biochem. Biophys. Res. Commun. 405, 134-139 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
N-terminal His6-tagged GluTR expressed in Escherichia coli
Acidithiobacillus ferrooxidans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
50000
-
SDS–PAGE of His6-tagged GluTR
Acidithiobacillus ferrooxidans
1.2.1.70
50490
-
MALDI-TOF mass spectrometry of His6-tagged GluTR
Acidithiobacillus ferrooxidans
1.2.1.70
104000
-
cross-linking of GluTR with glutaraldehyde
Acidithiobacillus ferrooxidans
1.2.1.70
148000
-
relative molecular mass of native recombinant GluTR after removal of His6-tag, determined by gel permeation chromatography
Acidithiobacillus ferrooxidans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Acidithiobacillus ferrooxidans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
by Ni2+-Sepharose affinity chromatography to near homogeneity
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
724174
Acidithiobacillus ferrooxidans
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
homodimer
2 * 50000, probably enyzme has an overall V shape that makes the protein migrate slower in gel permeation chromatography
Acidithiobacillus ferrooxidans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
enzyme shows Soret peak at 420 nm and a broad absorbance around 540 nm, data suggest that heme is bound preferentially to the dimeric form of GluTR
Acidithiobacillus ferrooxidans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
N-terminal His6-tagged GluTR expressed in Escherichia coli
Acidithiobacillus ferrooxidans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
enzyme shows Soret peak at 420 nm and a broad absorbance around 540 nm, data suggest that heme is bound preferentially to the dimeric form of GluTR
Acidithiobacillus ferrooxidans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
50000
-
SDS–PAGE of His6-tagged GluTR
Acidithiobacillus ferrooxidans
1.2.1.70
50490
-
MALDI-TOF mass spectrometry of His6-tagged GluTR
Acidithiobacillus ferrooxidans
1.2.1.70
104000
-
cross-linking of GluTR with glutaraldehyde
Acidithiobacillus ferrooxidans
1.2.1.70
148000
-
relative molecular mass of native recombinant GluTR after removal of His6-tag, determined by gel permeation chromatography
Acidithiobacillus ferrooxidans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
by Ni2+-Sepharose affinity chromatography to near homogeneity
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
724174
Acidithiobacillus ferrooxidans
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
homodimer
2 * 50000, probably enyzme has an overall V shape that makes the protein migrate slower in gel permeation chromatography
Acidithiobacillus ferrooxidans
General Information
EC Number
General Information
Commentary
Organism
1.2.1.70
metabolism
GluTR is the first enzyme committed to tetrapyrrole biosynthesis by the C5-pathway
Acidithiobacillus ferrooxidans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.2.1.70
metabolism
GluTR is the first enzyme committed to tetrapyrrole biosynthesis by the C5-pathway
Acidithiobacillus ferrooxidans