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Literature summary extracted from

  • Yoshinari, S.; Itoh, T.; Hallam, S.J.; DeLong, E.F.; Yokobori, S.; Yamagishi, A.; Oshima, T.; Kita, K.; Watanabe, Y.
    Archaeal pre-mRNA splicing: a connection to hetero-oligomeric splicing endonuclease (2006), Biochem. Biophys. Res. Commun., 346, 1024-1032.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.27.B1 for the reconstitution of the splicing endonuclease, STS047 with N-terminal S-tag and ST0358 with N-terminal His-tag are independently expressed in Escherichia coli Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.27.B1 cbf5 pre-mRNA fragment + H2O Sulfurisphaera tokodaii the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments. The enzyme catalyzes pre-mRNA splicing which is necessary to produce full-length and functional Cbf5 proteins ?
-
 ?
3.1.27.B1 cbf5 pre-mRNA fragment + H2O Sulfurisphaera tokodaii 7 the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments. The enzyme catalyzes pre-mRNA splicing which is necessary to produce full-length and functional Cbf5 proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.27.B1 Sulfurisphaera tokodaii Q975R3 and F9VMU0 Q975R3: subunit of splicing endonuclease, F9VMU0: subunit of splicing endonuclease
-
3.1.27.B1 Sulfurisphaera tokodaii 7 Q975R3 and F9VMU0 Q975R3: subunit of splicing endonuclease, F9VMU0: subunit of splicing endonuclease
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.27.B1 cbf5 pre-mRNA fragment + H2O the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments. The enzyme catalyzes pre-mRNA splicing which is necessary to produce full-length and functional Cbf5 proteins Sulfurisphaera tokodaii ?
-
 ?
3.1.27.B1 cbf5 pre-mRNA fragment + H2O the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments Sulfurisphaera tokodaii ?
-
 ?
3.1.27.B1 cbf5 pre-mRNA fragment + H2O the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments. The enzyme catalyzes pre-mRNA splicing which is necessary to produce full-length and functional Cbf5 proteins Sulfurisphaera tokodaii 7 ?
-
 ?
3.1.27.B1 cbf5 pre-mRNA fragment + H2O the enzyme cleaves at the exon-intron boundaries of cbf5 pre-mRNA fragments Sulfurisphaera tokodaii 7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.27.B1 heterooligomer
-
 Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.1.27.B1 splicing endonuclease EndA
-
 Sulfurisphaera tokodaii
3.1.27.B1 ST0358 subunit Sulfurisphaera tokodaii
3.1.27.B1 STK_03225 locus name, subunit Sulfurisphaera tokodaii
3.1.27.B1 STK_03580 locus name, subunit Sulfurisphaera tokodaii
3.1.27.B1 STS047 subunit Sulfurisphaera tokodaii