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Literature summary extracted from

  • Huneke, F.-U.; Nucci, R.; Cowan, D.
    Effect of water miscible organic solvents on kinetics of a thermostable beta-glycosidase (1999), Biocatal. Biotransform., 17, 251-267.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B26 acetonitrile maximal increase in enzyme activity at solvent concentrations of 15% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
3.2.1.B26 butan-2-one maximum increase in enzyme activity (1.5fold compared to activity in buffer) is measured at 5% (v/v) solvent concentrations with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
3.2.1.B26 methyl acetate 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside or 4-nitrophenyl beta-D-glucopyranoside Saccharolobus solfataricus
3.2.1.B26 methyl acetate highest activation at solvent concentrations of 5% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate Saccharolobus solfataricus
3.2.1.B26 tetrahydrofuran 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B26 1,4-dioxane 10% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-fucopyranoside Saccharolobus solfataricus
3.2.1.B26 butan-2-one 10-20 (v/v) Saccharolobus solfataricus
3.2.1.B26 D-gluconic acid lactone strong competitive inhibitor Saccharolobus solfataricus
3.2.1.B26 tetrahydrofuran 5% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-fucopyranoside Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.095
-
4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 0.2
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 0.83
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 1.14
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 2-nitrophenol + beta-D-galactopyranose
-
?
3.2.1.B26 4-nitrophenyl beta-D-fucopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-fucopyranose
-
?
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
?
3.2.1.B26 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 60
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 60
-
72 h, stable Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 462
-
4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 940
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 2374
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 2437
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 6.5
-
assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.B26 0.025
-
D-gluconic acid lactone pH 6.5, 60°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 2137
-
2-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 2860
-
4-nitrophenyl beta-D-galactopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 4700
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Saccharolobus solfataricus
3.2.1.B26 4863
-
4-nitrophenyl beta-D-fucopyranoside pH 6.5, 60°C Saccharolobus solfataricus