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Literature summary extracted from

  • Al-Onazi, M.; Al-Dahain, S.; El-Ansary, A.; Marraiki, N.
    Isolation and characterization of Thielaviopsis paradoxa L-alanine dehydrogenase (2011), Asian J. Appl. Sci., 4, 702-711.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.1 DTT 31% activation at 10 mM Thielaviopsis paradoxa
1.4.1.1 glutathione 16.5% activation at 10 mM Thielaviopsis paradoxa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Co2+ 17% inhibition at 10 mM, 23.75% at 20 mM Thielaviopsis paradoxa
1.4.1.1 iodoacetate 23.75% inhibition at 10 mM Thielaviopsis paradoxa
1.4.1.1 Zn2+ 57% inhibition at 10 mM, 72.75% at 20 mM Thielaviopsis paradoxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.197
-
 NADH pH 8.5, 60°C Thielaviopsis paradoxa
1.4.1.1 0.274
-
 NAD+ pH 9.5, 60°C Thielaviopsis paradoxa
1.4.1.1 1.35
-
 L-alanine pH 9.5, 60°C Thielaviopsis paradoxa
1.4.1.1 3 3.5 NH3 pH 8.5, 60°C Thielaviopsis paradoxa
1.4.1.1 8.16
-
 pyruvate pH 8.5, 60°C Thielaviopsis paradoxa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 DL-alanine + H2O + NAD+ Thielaviopsis paradoxa
-
 pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 glycine + H2O + NAD+ Thielaviopsis paradoxa
-
 glyoxylate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+ Thielaviopsis paradoxa
-
 pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-isoleucine + H2O + NAD+ Thielaviopsis paradoxa
-
 2-oxo-3-methylpentanoate + NH3 + NADH + H+
-
 r
1.4.1.1 L-serine + H2O + NAD+ Thielaviopsis paradoxa
-
 3-hydroxypyruvate + NH3 + NADH + NH3 + NADH + H+
-
 r
1.4.1.1 L-threonine + H2O + NAD+ Thielaviopsis paradoxa
-
 3-hydroxy-2-oxobutyrate + NH3 + NADH + H+
-
 r
1.4.1.1 additional information Thielaviopsis paradoxa substrate specifiicty, overview. The enzyme is also active with substrates DL-alanine, L-serine, L-isoleucine, L-threonine, and glycine, with L-serine giving the highest activity rate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Thielaviopsis paradoxa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 native enzyme Thielaviopsis paradoxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 DL-alanine + H2O + NAD+
-
 Thielaviopsis paradoxa pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 glycine + H2O + NAD+
-
 Thielaviopsis paradoxa glyoxylate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+
-
 Thielaviopsis paradoxa pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-isoleucine + H2O + NAD+
-
 Thielaviopsis paradoxa 2-oxo-3-methylpentanoate + NH3 + NADH + H+
-
 r
1.4.1.1 L-serine + H2O + NAD+
-
 Thielaviopsis paradoxa 3-hydroxypyruvate + NH3 + NADH + NH3 + NADH + H+
-
 r
1.4.1.1 L-threonine + H2O + NAD+
-
 Thielaviopsis paradoxa 3-hydroxy-2-oxobutyrate + NH3 + NADH + H+
-
 r
1.4.1.1 additional information substrate specifiicty, overview. The enzyme is also active with substrates DL-alanine, L-serine, L-isoleucine, L-threonine, and glycine, with L-serine giving the highest activity rate Thielaviopsis paradoxa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALADH
-
 Thielaviopsis paradoxa
1.4.1.1 L-alanine dehydrogenase
-
 Thielaviopsis paradoxa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 60
-
 assay at Thielaviopsis paradoxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8.5
-
 amination of pyruuvate Thielaviopsis paradoxa
1.4.1.1 9.5
-
 deamination of L-alanine Thielaviopsis paradoxa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Thielaviopsis paradoxa
1.4.1.1 NADH
-
 Thielaviopsis paradoxa

General Information

EC Number General Information Comment Organism
1.4.1.1 physiological function the enzyme plays an important role in the carbon and nitrogen metabolism of microorganisms, it iis a key factor in assimilation of L-Ala as energy source through TCA cycle Thielaviopsis paradoxa