BRENDA - Enzyme Database show

Oligomerization of Sulfolobus solfataricus signature amidase is promoted by acidic pH and high temperature

D'Abusco, A.S.; Casadio, R.; Tasco, G.; Giangiacomo, L.; Giartosio, A.; Calamia, V.; Di Marco, S.; Chiaraluce, R.; Consalvi, V.; Scandurra, R.; Politi, L.; Archaea 1, 411-423 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.4
expression in Escherichia coli
Saccharolobus solfataricus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.4
Y41C
no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased
Saccharolobus solfataricus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.1
-
2-toluamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.1
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.2
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.2
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.2
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.3
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.3
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.3
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.4
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.5
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.6
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.8
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.9
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
3
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
3.2
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
4.5
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.5
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
7
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
10
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
14
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
22
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
37
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
55784
-
x * 55784, calculated from amino acid sequence
Saccharolobus solfataricus
3.5.1.4
83000
-
dimeric enzyme form, gel filtration
Saccharolobus solfataricus
3.5.1.4
400000
-
octameric enzyme form, gel filtration
Saccharolobus solfataricus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Saccharolobus solfataricus
P95896
-
-
3.5.1.4
Saccharolobus solfataricus P2
P95896
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.4
-
Saccharolobus solfataricus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
2-toluamide + H2O
-
724144
Saccharolobus solfataricus
2-toluic acid + NH3
-
-
-
?
3.5.1.4
3-phenylpropionamide + H2O
-
724144
Saccharolobus solfataricus
3-phenylpropionate + NH3
-
-
-
?
3.5.1.4
4-hydroxybenzamide + H2O
-
724144
Saccharolobus solfataricus
4-hydroxybenzoate + NH3
-
-
-
?
3.5.1.4
4-methylbenzamide + H2O
-
724144
Saccharolobus solfataricus
4-methylbenzoic acid + NH3
-
-
-
?
3.5.1.4
4-nitrobenzamide + H2O
-
724144
Saccharolobus solfataricus
4-nitrobenzoate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
724144
Saccharolobus solfataricus
acetic acid + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
724144
Saccharolobus solfataricus P2
acetic acid + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
724144
Saccharolobus solfataricus
benzoate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
724144
Saccharolobus solfataricus
butyrate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
724144
Saccharolobus solfataricus P2
butyrate + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
724144
Saccharolobus solfataricus
hexanoate + NH3
-
-
-
?
3.5.1.4
indole-3-acetamide + H2O
-
724144
Saccharolobus solfataricus
indole-3-acetate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
724144
Saccharolobus solfataricus
isobutyrate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
724144
Saccharolobus solfataricus P2
isobutyrate + NH3
-
-
-
?
3.5.1.4
methacrylamide + H2O
-
724144
Saccharolobus solfataricus
methacrylate + NH3
-
-
-
-
3.5.1.4
nicotinamide + H2O
-
724144
Saccharolobus solfataricus
nicotinic acid + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
724144
Saccharolobus solfataricus
pentanoate + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
724144
Saccharolobus solfataricus P2
pentanoate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
724144
Saccharolobus solfataricus
propionic acid + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
724144
Saccharolobus solfataricus P2
propionic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 55784, calculated from amino acid sequence
Saccharolobus solfataricus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
assay at
Saccharolobus solfataricus
3.5.1.4
95
-
-
Saccharolobus solfataricus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
additional information
-
thermal unfolding is not reversible
Saccharolobus solfataricus
3.5.1.4
70
-
thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h
Saccharolobus solfataricus
3.5.1.4
80
-
half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0
Saccharolobus solfataricus
3.5.1.4
84
-
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C
Saccharolobus solfataricus
3.5.1.4
90
-
half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0
Saccharolobus solfataricus
3.5.1.4
94
-
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C
Saccharolobus solfataricus
3.5.1.4
97
-
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C
Saccharolobus solfataricus
3.5.1.4
104
-
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C
Saccharolobus solfataricus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.4
-
2-toluamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.8
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.8
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.2
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
1.9
-
4-hydroxybenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.1
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.2
-
indol-3-acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.2
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
3
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
4.8
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.4
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.7
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
7.2
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
12.2
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
14.2
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
15.3
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
17.5
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
18
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
19.5
-
hexanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
21.6
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
26.8
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
28.6
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
28.9
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
30.1
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
34.3
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
48.9
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
58.4
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
82.9
-
Propionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
99.4
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
5
-
at 70°C, assay at
Saccharolobus solfataricus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
3.5
8.5
pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
expression in Escherichia coli
Saccharolobus solfataricus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.4
Y41C
no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased
Saccharolobus solfataricus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.1
-
2-toluamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.1
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.2
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.2
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.2
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.3
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.3
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.3
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.4
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.5
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.6
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.8
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.9
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
3
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
3.2
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
4.5
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.5
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
7
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
10
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
14
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
22
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
37
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
55784
-
x * 55784, calculated from amino acid sequence
Saccharolobus solfataricus
3.5.1.4
83000
-
dimeric enzyme form, gel filtration
Saccharolobus solfataricus
3.5.1.4
400000
-
octameric enzyme form, gel filtration
Saccharolobus solfataricus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
-
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
2-toluamide + H2O
-
724144
Saccharolobus solfataricus
2-toluic acid + NH3
-
-
-
?
3.5.1.4
3-phenylpropionamide + H2O
-
724144
Saccharolobus solfataricus
3-phenylpropionate + NH3
-
-
-
?
3.5.1.4
4-hydroxybenzamide + H2O
-
724144
Saccharolobus solfataricus
4-hydroxybenzoate + NH3
-
-
-
?
3.5.1.4
4-methylbenzamide + H2O
-
724144
Saccharolobus solfataricus
4-methylbenzoic acid + NH3
-
-
-
?
3.5.1.4
4-nitrobenzamide + H2O
-
724144
Saccharolobus solfataricus
4-nitrobenzoate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
724144
Saccharolobus solfataricus
acetic acid + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
724144
Saccharolobus solfataricus P2
acetic acid + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
724144
Saccharolobus solfataricus
benzoate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
724144
Saccharolobus solfataricus
butyrate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
724144
Saccharolobus solfataricus P2
butyrate + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
724144
Saccharolobus solfataricus
hexanoate + NH3
-
-
-
?
3.5.1.4
indole-3-acetamide + H2O
-
724144
Saccharolobus solfataricus
indole-3-acetate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
724144
Saccharolobus solfataricus
isobutyrate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
-
724144
Saccharolobus solfataricus P2
isobutyrate + NH3
-
-
-
?
3.5.1.4
methacrylamide + H2O
-
724144
Saccharolobus solfataricus
methacrylate + NH3
-
-
-
-
3.5.1.4
nicotinamide + H2O
-
724144
Saccharolobus solfataricus
nicotinic acid + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
724144
Saccharolobus solfataricus
pentanoate + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
724144
Saccharolobus solfataricus P2
pentanoate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
724144
Saccharolobus solfataricus
propionic acid + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
724144
Saccharolobus solfataricus P2
propionic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 55784, calculated from amino acid sequence
Saccharolobus solfataricus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
assay at
Saccharolobus solfataricus
3.5.1.4
95
-
-
Saccharolobus solfataricus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
additional information
-
thermal unfolding is not reversible
Saccharolobus solfataricus
3.5.1.4
70
-
thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h
Saccharolobus solfataricus
3.5.1.4
80
-
half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0
Saccharolobus solfataricus
3.5.1.4
84
-
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C
Saccharolobus solfataricus
3.5.1.4
90
-
half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0
Saccharolobus solfataricus
3.5.1.4
94
-
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C
Saccharolobus solfataricus
3.5.1.4
97
-
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C
Saccharolobus solfataricus
3.5.1.4
104
-
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C
Saccharolobus solfataricus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.4
-
2-toluamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
0.8
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
0.8
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.2
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
1.9
-
4-hydroxybenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.1
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.2
-
indol-3-acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.2
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.5
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
3
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
4.8
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.4
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
6.7
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
7.2
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
12.2
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
14.2
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
15.3
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
17.5
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
18
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
19.5
-
hexanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
21.6
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
26.8
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
28.6
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
28.9
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
30.1
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
34.3
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
48.9
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
58.4
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
82.9
-
Propionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
99.4
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
5
-
at 70°C, assay at
Saccharolobus solfataricus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
3.5
8.5
pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity
Saccharolobus solfataricus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.81
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
1.31
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.45
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.75
-
indol-3-acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.26
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.33
-
hexanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.8
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
5.1
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
5.47
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
5.5
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
6
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
6.99
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
7.2
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
7.44
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
8.29
-
Propionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
8.33
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
8.98
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
9.94
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
10.7
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
12
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
16
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
19
-
4-hydroxybenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
19
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
21
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
47.3
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
61
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.81
-
acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
1.31
-
acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
1.5
-
indol-3-acetamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
2.45
-
butyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
2.75
-
indol-3-acetamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4
-
2-toluamide
pH 5.0, 70°C, mutant enzyme Y41C; pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4
-
4-nitrobenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.26
-
nicotinamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.33
-
hexanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
4.8
-
hexanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
5.1
-
3-phenylpropionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
5.47
-
butyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
5.5
-
nicotinamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
6
-
metacrylamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
6.99
-
Isobutyramide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
7.2
-
3-phenylpropionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
7.44
-
Benzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
8.29
-
Propionamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
8.33
-
4-nitrobenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
8.98
-
Isobutyramide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
9.94
-
Propionamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
10.7
-
Pentanamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
12
-
Benzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
16
-
metacrylamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
19
-
4-hydroxybenzamide
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus
3.5.1.4
19
-
Pentanamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
21
-
4-hydroxybenzamide
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
47.3
-
cyclomaltohexaose
pH 5.0, 70°C, mutant enzyme Y41C
Saccharolobus solfataricus
3.5.1.4
61
-
cyclomaltohexaose
pH 5.0, 70°C, wild-type enzyme
Saccharolobus solfataricus