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Literature summary extracted from

  • D'Abusco, A.S.; Casadio, R.; Tasco, G.; Giangiacomo, L.; Giartosio, A.; Calamia, V.; Di Marco, S.; Chiaraluce, R.; Consalvi, V.; Scandurra, R.; Politi, L.
    Oligomerization of Sulfolobus solfataricus signature amidase is promoted by acidic pH and high temperature (2005), Archaea, 1, 411-423.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.4 Y41C no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.1
-
4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.1
-
2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.1
-
4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.2
-
cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.2
-
4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.2
-
2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.3
-
metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.3
-
cyclomaltohexaose pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.3
-
4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.4
-
nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.5
-
metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.6
-
Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.8
-
indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.8
-
indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 0.9
-
Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 1.5
-
nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 1.5
-
Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 2.5
-
Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.5
-
3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 3
-
3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 3.2
-
butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 4.5
-
hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4.5
-
hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 6.5
-
Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 7
-
Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 10
-
Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 10
-
Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 14
-
butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 22
-
acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 37
-
acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 55784
-
x * 55784, calculated from amino acid sequence Saccharolobus solfataricus
3.5.1.4 83000
-
dimeric enzyme form, gel filtration Saccharolobus solfataricus
3.5.1.4 400000
-
octameric enzyme form, gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Saccharolobus solfataricus P95896
-
-
3.5.1.4 Saccharolobus solfataricus P2 P95896
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 2-toluamide + H2O
-
Saccharolobus solfataricus 2-toluic acid + NH3
-
?
3.5.1.4 3-phenylpropionamide + H2O
-
Saccharolobus solfataricus 3-phenylpropionate + NH3
-
?
3.5.1.4 4-hydroxybenzamide + H2O
-
Saccharolobus solfataricus 4-hydroxybenzoate + NH3
-
?
3.5.1.4 4-methylbenzamide + H2O
-
Saccharolobus solfataricus 4-methylbenzoic acid + NH3
-
?
3.5.1.4 4-nitrobenzamide + H2O
-
Saccharolobus solfataricus 4-nitrobenzoate + NH3
-
?
3.5.1.4 4-toluamide + H2O
-
Saccharolobus solfataricus 4-toluic acid + NH3
-
?
3.5.1.4 acetamide + H2O
-
Saccharolobus solfataricus acetic acid + NH3
-
?
3.5.1.4 acetamide + H2O
-
Saccharolobus solfataricus P2 acetic acid + NH3
-
?
3.5.1.4 benzamide + H2O
-
Saccharolobus solfataricus benzoate + NH3
-
?
3.5.1.4 butyramide + H2O
-
Saccharolobus solfataricus butyrate + NH3
-
?
3.5.1.4 butyramide + H2O
-
Saccharolobus solfataricus P2 butyrate + NH3
-
?
3.5.1.4 hexanamide + H2O
-
Saccharolobus solfataricus hexanoate + NH3
-
?
3.5.1.4 indole-3-acetamide + H2O
-
Saccharolobus solfataricus indole-3-acetate + NH3
-
?
3.5.1.4 isobutyramide + H2O
-
Saccharolobus solfataricus isobutyrate + NH3
-
?
3.5.1.4 isobutyramide + H2O
-
Saccharolobus solfataricus P2 isobutyrate + NH3
-
?
3.5.1.4 methacrylamide + H2O
-
Saccharolobus solfataricus methacrylate + NH3
-
?
3.5.1.4 nicotinamide + H2O
-
Saccharolobus solfataricus nicotinic acid + NH3
-
?
3.5.1.4 pentanamide + H2O
-
Saccharolobus solfataricus pentanoate + NH3
-
?
3.5.1.4 pentanamide + H2O
-
Saccharolobus solfataricus P2 pentanoate + NH3
-
?
3.5.1.4 propionamide + H2O
-
Saccharolobus solfataricus propionic acid + NH3
-
?
3.5.1.4 propionamide + H2O
-
Saccharolobus solfataricus P2 propionic acid + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 ? x * 55784, calculated from amino acid sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.4 signature amidase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 70
-
assay at Saccharolobus solfataricus
3.5.1.4 95
-
-
Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.4 additional information
-
thermal unfolding is not reversible Saccharolobus solfataricus
3.5.1.4 70
-
thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h Saccharolobus solfataricus
3.5.1.4 80
-
half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0 Saccharolobus solfataricus
3.5.1.4 84
-
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C Saccharolobus solfataricus
3.5.1.4 90
-
half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0 Saccharolobus solfataricus
3.5.1.4 94
-
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C Saccharolobus solfataricus
3.5.1.4 97
-
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C Saccharolobus solfataricus
3.5.1.4 104
-
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.4
-
2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.8
-
4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.8
-
2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 1.2
-
indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 1.9
-
4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.1
-
4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 2.2
-
indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.2
-
nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 2.5
-
4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 3
-
metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 4.8
-
metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 6.4
-
nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 6.7
-
Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 7.2
-
Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 12.2
-
4-toluamide wild type enzyme Saccharolobus solfataricus
3.5.1.4 12.2
-
4-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 14.2
-
4-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 14.2
-
4-toluamide mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 15.3
-
3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 17.5
-
butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 18
-
3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 19.5
-
hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 21.6
-
hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 26.8
-
Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 28.6
-
Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 28.9
-
acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 30.1
-
acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 34.3
-
butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 48.9
-
Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 58.4
-
Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 82.9
-
Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 99.4
-
Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 5
-
at 70°C, assay at Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 3.5 8.5 pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.4 0.81
-
acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 1.31
-
acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 1.5
-
indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 2.45
-
butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.75
-
indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4
-
2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4
-
4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4
-
2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 4.26
-
nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4.33
-
hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4.8
-
hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 5.1
-
3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 5.47
-
butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 5.5
-
nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 6
-
metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 6.99
-
Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 7.2
-
3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 7.44
-
Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 8.29
-
Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 8.33
-
4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 8.98
-
Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 9.94
-
Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 10.7
-
Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 12
-
Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 16
-
metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 19
-
4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 19
-
Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 21
-
4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 47.3
-
cyclomaltohexaose pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3.5.1.4 61
-
cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus