EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.40 | 0.04 | - |
reduced coenzyme F420 | pH 8.0, 30°C | Methanogenium organophilum | |
1.5.1.40 | 0.05 | - |
NADPH | pH 6.0, 30°C | Methanogenium organophilum | |
1.5.1.40 | 0.1 | - |
NADP+ | pH 8.0, 30°C | Methanogenium organophilum | |
1.5.1.40 | 0.1 | - |
oxidized coenzyme F420 | pH 6.0, 30°C | Methanogenium organophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 28000 | - |
x * 28000, SDS-PAGE | Methanogenium organophilum |
1.5.1.40 | 90000 | - |
gradient gel electrophoresis under nondenaturating conditions | Methanogenium organophilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.40 | Methanobacterium palustre | - |
- |
- |
1.5.1.40 | Methanobacterium palustre DSM 3108 | - |
- |
- |
1.5.1.40 | Methanoculleus thermophilus | - |
- |
- |
1.5.1.40 | Methanoculleus thermophilus DSM 3915 | - |
- |
- |
1.5.1.40 | Methanofollis liminatans | - |
- |
- |
1.5.1.40 | Methanofollis liminatans DSM 4140 | - |
- |
- |
1.5.1.40 | Methanogenium organophilum | P80951 | - |
- |
1.5.1.40 | Methanogenium organophilum DSM 3596 | P80951 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.5.1.40 | stable under oxic conditions | Methanogenium organophilum |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.40 | - |
Methanogenium organophilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 0.04 | - |
pH 8.0, 55°C, cell extract | Methanoculleus thermophilus |
1.5.1.40 | 0.06 | - |
pH 8.0, 37°C, cell extract | Methanofollis liminatans |
1.5.1.40 | 0.3 | - |
pH 8.0, 37°C, cell extract | Methanobacterium palustre |
1.5.1.40 | 15.1 | - |
pH 8.0, 30°C, cell extract | Methanogenium organophilum |
1.5.1.40 | 998 | - |
pH 8.0, 30°C, purified enzyme | Methanogenium organophilum |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.40 | 4°C or -20°C, enzyme solution is stable for several weeks | Methanogenium organophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans DSM 4140 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum DSM 3596 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre DSM 3108 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus DSM 3915 | oxidized coenzyme F420 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.40 | ? | x * 28000, SDS-PAGE | Methanogenium organophilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.40 | F420-dependent NADP reductase | - |
Methanoculleus thermophilus |
1.5.1.40 | F420-dependent NADP reductase | - |
Methanofollis liminatans |
1.5.1.40 | F420-dependent NADP reductase | - |
Methanobacterium palustre |
1.5.1.40 | F420-dependent NADP reductase | - |
Methanogenium organophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 34 | - |
- |
Methanogenium organophilum |
1.5.1.40 | 37 | - |
assay at | Methanofollis liminatans |
1.5.1.40 | 37 | - |
assay at | Methanobacterium palustre |
1.5.1.40 | 55 | - |
assay at | Methanoculleus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 5.5 | - |
pH optimum for reduction of oxidized coenzyme F420 with NADPH | Methanogenium organophilum |
1.5.1.40 | 9.2 | - |
pH optimum for reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 7.5 | 10 | pH 7.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity, reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |