EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.8 | expression in Escherichia coli | Methylophaga sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.8 | W47A | insoluble inactive protein | Methylophaga sp. |
1.14.13.8 | W47F | soluble and active protein. The spectrum of the flavin displays a redshift, the kcat values for NADPH, trimethylamine, and methimazole, show a 5-8fold decrease, and primary kinetic isotope effect values are higher than in wild-type. Mutant displays reduced flexibility in active site residues and, in particular, the nicotinamide moiety of NADP+ | Methylophaga sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.8 | 0.0015 | - |
trimethylamine | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.0026 | - |
trimethylamine | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.0041 | - |
NADPH | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.0043 | - |
NADPH | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.019 | - |
O2 | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.037 | - |
Methimazole | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.042 | - |
O2 | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.163 | - |
Methimazole | wild-type, pH 8.5, 25°C | Methylophaga sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.8 | Methylophaga sp. | - |
- |
- |
1.14.13.8 | Methylophaga sp. SK1 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.8 | methimazole + NADPH + H+ + O2 | - |
Methylophaga sp. | methimazole N-oxide + NADP+ + H2O | - |
? | |
1.14.13.8 | methimazole + NADPH + H+ + O2 | - |
Methylophaga sp. SK1 | methimazole N-oxide + NADP+ + H2O | - |
? | |
1.14.13.8 | trimethylamine + NADPH + H+ + O2 | - |
Methylophaga sp. | trimethylamine N-oxide + NADP+ + H2O | - |
? | |
1.14.13.8 | trimethylamine + NADPH + H+ + O2 | - |
Methylophaga sp. SK1 | trimethylamine N-oxide + NADP+ + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.8 | 0.67 | - |
Methimazole | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 0.92 | - |
NADPH | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 1.25 | - |
trimethylamine | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 2.7 | - |
Methimazole | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 2.7 | - |
O2 | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 6.1 | - |
trimethylamine | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 7.3 | - |
NADPH | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 7.7 | - |
O2 | wild-type, pH 8.5, 25°C | Methylophaga sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.8 | 16 | - |
Methimazole | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 18 | - |
Methimazole | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 60 | - |
O2 | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 210 | - |
NADPH | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 410 | - |
O2 | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 800 | - |
trimethylamine | mutant W47F, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 1800 | - |
NADPH | wild-type, pH 8.5, 25°C | Methylophaga sp. | |
1.14.13.8 | 2300 | - |
trimethylamine | wild-type, pH 8.5, 25°C | Methylophaga sp. |