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Literature summary extracted from

  • Sarker, L.S.; Galata, M.; Demissie, Z.A.; Mahmoud, S.S.
    Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia (2012), Arch. Biochem. Biophys., 528, 163-170.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.227 biotechnology the gene encoding the borneol dhdrogenase is a target for metabolic engineering for improvement of essential oil production Lavandula x intermedia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.198 expression in Escherichia coli Lavandula x intermedia
1.1.1.227 gene LiBDH, DNA and amino acid sequence determination and analysis, phylogenetic tree, gene expression is regulated in a tissue-specific manner, expression of the His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLysS Lavandula x intermedia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.198 0.053
-
 (+)-borneol pH 8.0, 32°C Lavandula x intermedia
1.1.1.227 additional information
-
 additional information Michaelis-Menten kinetics, overview Lavandula x intermedia
1.1.1.227 0.053
-
 (-)-borneol pH 8.0, 30°C, recombinant enzyme Lavandula x intermedia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.198 27500
-
 x * 27500, calculated Lavandula x intermedia
1.1.1.227 27500
-
 x * 27500, about, sequence calculation Lavandula x intermedia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.227 (-)-borneol + NAD+ Lavandula x intermedia
-
 (-)-camphor + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.198 Lavandula x intermedia K4N0V2
-
-
1.1.1.227 Lavandula x intermedia
-
 gene LiBDH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.227 recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography Lavandula x intermedia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.198 flower transcripts are specifically expressed in glandular trichomes of mature flowers Lavandula x intermedia
-
1.1.1.198 trichome transcripts are specifically expressed in glandular trichomes of mature flowers Lavandula x intermedia
-
1.1.1.227 flower specifically in glandular trichomes of mature flowers Lavandula x intermedia
-
1.1.1.227 additional information the gene exppression is regulated in a tissue-specific manner Lavandula x intermedia
-
1.1.1.227 trichome
-
 Lavandula x intermedia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+
-
 Lavandula x intermedia (+)-camphor + NADH + H+ camphor is the only product ir
1.1.1.198 additional information no substrate: 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, perillyl alcohol, and farnesol. The reverse reduction assay in which camphor is used as a substrate and NADH as a cofactor, does not produce detectable amounts of borneol or other products Lavandula x intermedia ?
-
 ?
1.1.1.227 (-)-borneol + NAD+
-
 Lavandula x intermedia (-)-camphor + NADH + H+
-
 ir
1.1.1.227 additional information no activity with other monoterpenes, e.g. alpha terpineol, 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, and perillyl alcohol, and sesquiterpene farnesol, or with menthol, overview Lavandula x intermedia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.198 ? x * 27500, calculated Lavandula x intermedia
1.1.1.227 ? x * 27500, about, sequence calculation Lavandula x intermedia

Synonyms

EC Number Synonyms Comment Organism
1.1.1.227 borneol dehydrogenase
-
 Lavandula x intermedia
1.1.1.227 LiBDH
-
 Lavandula x intermedia
1.1.1.227 LiSDR-2
-
 Lavandula x intermedia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.227 32
-
-
 Lavandula x intermedia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.198 0.0004
-
 (+)-borneol pH 8.0, 32°C Lavandula x intermedia
1.1.1.227 0.0004
-
 (-)-borneol pH 8.0, 30°C, recombinant enzyme Lavandula x intermedia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.227 8
-
-
 Lavandula x intermedia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.198 additional information no cofactor: NADP+ Lavandula x intermedia
1.1.1.198 NAD+
-
 Lavandula x intermedia
1.1.1.227 additional information no activity with NADP+ Lavandula x intermedia
1.1.1.227 NAD+ preferred cofactor Lavandula x intermedia

General Information

EC Number General Information Comment Organism
1.1.1.227 evolution the enzyme is a member of the SDR superfamily of enzymes Lavandula x intermedia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.227 0.0075
-
 (-)-borneol pH 8.0, 30°C, recombinant enzyme Lavandula x intermedia