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Literature summary extracted from

  • Santos, A.; Mendes, S.; Brissos, V.; Martins, L.
    New dye-decolorizing peroxidases from Bacillus subtilis and Pseudomonas putida MET94: towards biotechnological applications (2014), Appl. Microbiol. Biotechnol., 98, 253-2065.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expression in Escherichia coli Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.009
-
 H2O2 pH 4, 25°C Bacillus subtilis
1.11.1.19 0.157
-
 Reactive Blue 5 pH 4, 25°C Bacillus subtilis
1.11.1.19 0.166
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4, 25°C Bacillus subtilis
1.11.1.19 0.385
-
 Mordant black 9 pH 4, 25°C Bacillus subtilis
1.11.1.19 0.444
-
 Acid Blue 62 pH 4, 25°C Bacillus subtilis
1.11.1.19 0.499
-
 guaiacol pH 4, 25°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.19 48000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Bacillus subtilis ? + H2O
-
 ?
1.11.1.19 Acid Blue 62 + H2O2 + H+
-
 Bacillus subtilis oxidized Acid Blue 62 + H2O
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Bacillus subtilis oxidized guaiacol + H2O
-
 ?
1.11.1.19 Mordant Black 9 + H2O2 + H+
-
 Bacillus subtilis oxidized Mordant Black 9 + H2O
-
 ?
1.11.1.19 additional information poor activity with Mn2+, no substrates: Fe2+, syringaldehyde, acettosyringone, veratryl alcohol Bacillus subtilis ?
-
 ?
1.11.1.19 Reactive Blue 5 + H2O2 + H+
-
 Bacillus subtilis oxidized Reactive Blue 5 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 monomer 1 * 48000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.19 20 30
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme enzyme shows the typical Soret band, heme content is 0.5 mol per mol of protein Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.3
-
 guaiacol pH 4, 25°C Bacillus subtilis
1.11.1.19 10
-
 Mordant black 9 pH 4, 25°C Bacillus subtilis
1.11.1.19 20
-
 Acid Blue 62 pH 4, 25°C Bacillus subtilis
1.11.1.19 50
-
 Reactive Blue 5 pH 4, 25°C Bacillus subtilis
1.11.1.19 2000
-
 H2O2 pH 4, 25°C Bacillus subtilis