EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.99.6 | expressed in Escherichia coli Rosetta (DE3) cells | Ureibacillus thermosphaericus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 32.3 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.99.6 | Ureibacillus thermosphaericus | - |
- |
- |
1.4.99.6 | Ureibacillus thermosphaericus A1 (NBRC 108682) | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.99.6 | Sepharose column chromatography, and gel filtration | Ureibacillus thermosphaericus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.6 | (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-isoleucine + H2O + NADP+ | yield of 45% after incubation for 1 h at 50 °C | ? | |
1.4.99.6 | (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-isoleucine + H2O + NADP+ | yield of 45% after incubation for 1 h at 50 °C | ? | |
1.4.99.6 | 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-valine + H2O + NADP+ | yield of 83% after incubation for 1 h at 50 °C | ? | |
1.4.99.6 | 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-valine + H2O + NADP+ | yield of 83% after incubation for 1 h at 50 °C | ? | |
1.4.99.6 | 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-leucine + H2O + NADP+ | yield of 91% after incubation for 1 h at 50 °C | ? | |
1.4.99.6 | 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-leucine + H2O + NADP+ | yield of 91% after incubation for 1 h at 50 °C | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.99.6 | D-amino acid dehydrogenase | - |
Ureibacillus thermosphaericus |
1.4.99.6 | DAADH | - |
Ureibacillus thermosphaericus |
1.4.99.6 | NADP+-dependent D-amino acid dehydrogenase | - |
Ureibacillus thermosphaericus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 65 | - |
temperature optimum for D-leucine synthesis | Ureibacillus thermosphaericus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 5700 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 10.5 | - |
pH-optimum for D-leucine synthesisin glycine-KOH buffer | Ureibacillus thermosphaericus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.6 | NADP+ | the yield is highest in the presence of 0.5 mM NADP+ | Ureibacillus thermosphaericus | |
1.4.99.6 | NADPH | dependent on | Ureibacillus thermosphaericus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 180 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus |