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Literature summary extracted from

  • Akita, H.; Suzuki, H.; Doi, K.; Ohshima, T.
    Efficient synthesis of D-branched-chain amino acids and their labeled compounds with stable isotopes using D-amino acid dehydrogenase (2014), Appl. Microbiol. Biotechnol., 98, 1135-1143.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.99.6 expressed in Escherichia coli Rosetta (DE3) cells Ureibacillus thermosphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 32.3
-
2-oxo-4-methylvaleric acid at pH 10.5 and 50°C Ureibacillus thermosphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Ureibacillus thermosphaericus
-
-
-
1.4.99.6 Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.99.6 Sepharose column chromatography, and gel filtration Ureibacillus thermosphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus D-isoleucine + H2O + NADP+ yield of 45% after incubation for 1 h at 50 °C ?
1.4.99.6 (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus A1 (NBRC 108682) D-isoleucine + H2O + NADP+ yield of 45% after incubation for 1 h at 50 °C ?
1.4.99.6 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus D-valine + H2O + NADP+ yield of 83% after incubation for 1 h at 50 °C ?
1.4.99.6 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus A1 (NBRC 108682) D-valine + H2O + NADP+ yield of 83% after incubation for 1 h at 50 °C ?
1.4.99.6 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus D-leucine + H2O + NADP+ yield of 91% after incubation for 1 h at 50 °C ?
1.4.99.6 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+
-
Ureibacillus thermosphaericus A1 (NBRC 108682) D-leucine + H2O + NADP+ yield of 91% after incubation for 1 h at 50 °C ?

Synonyms

EC Number Synonyms Comment Organism
1.4.99.6 D-amino acid dehydrogenase
-
Ureibacillus thermosphaericus
1.4.99.6 DAADH
-
Ureibacillus thermosphaericus
1.4.99.6 NADP+-dependent D-amino acid dehydrogenase
-
Ureibacillus thermosphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.99.6 65
-
temperature optimum for D-leucine synthesis Ureibacillus thermosphaericus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.99.6 5700
-
2-oxo-4-methylvaleric acid at pH 10.5 and 50°C Ureibacillus thermosphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.99.6 10.5
-
pH-optimum for D-leucine synthesisin glycine-KOH buffer Ureibacillus thermosphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.99.6 NADP+ the yield is highest in the presence of 0.5 mM NADP+ Ureibacillus thermosphaericus
1.4.99.6 NADPH dependent on Ureibacillus thermosphaericus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.99.6 180
-
2-oxo-4-methylvaleric acid at pH 10.5 and 50°C Ureibacillus thermosphaericus