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Literature summary extracted from

  • Girhard, M.; Tieves, F.; Weber, E.; Smit, M.S.; Urlacher, V.B.
    Cytochrome P450 reductase from Candida apicola: versatile redox partner for bacterial P450s (2013), Appl. Microbiol. Biotechnol., 97, 1625-1635.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 gene cpr, DNA and amino acid sequence determination and analysis, expression of the N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, in Escherichia coli strain BL21 (DE3) Starmerella apicola

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.4 additional information truncation f the N-terminal putative membrane anchor leads to increased activity in reducing cytochrome c as compared to the wild-type enzyme, overview Starmerella apicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 additional information
-
 additional information Michaelis-Menten kinetics Starmerella apicola
1.6.2.4 0.0138
-
 cytochrome c pH 7.5, 25°C Starmerella apicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 76068
-
 x * 76068, sequence calculation Starmerella apicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 NADPH + H+ + cytochrome c Starmerella apicola
-
 NADP+ + reduced cytochrome c
-
 ?
1.6.2.4 NADPH + H+ + cytochrome c Starmerella apicola ATCC 96134
-
 NADP+ + reduced cytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Starmerella apicola H2ER12 gene cpr
-
1.6.2.4 Starmerella apicola ATCC 96134 H2ER12 gene cpr
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 recombinant N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 98% purity Starmerella apicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 NADPH + H+ + cytochrome c
-
 Starmerella apicola NADP+ + reduced cytochrome c
-
 ?
1.6.2.4 NADPH + H+ + cytochrome c
-
 Starmerella apicola ATCC 96134 NADP+ + reduced cytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 76068, sequence calculation Starmerella apicola

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Starmerella apicola
1.6.2.4 cytochrome P450-diflavin reductase
-
 Starmerella apicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.2.4 25
-
 assay at Starmerella apicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 31.92
-
 cytochrome c pH 7.5, 25°C Starmerella apicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.5
-
 assay at Starmerella apicola

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Starmerella apicola
1.6.2.4 FMN
-
 Starmerella apicola
1.6.2.4 additional information CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain Starmerella apicola
1.6.2.4 NADPH
-
 Starmerella apicola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.2.4 Starmerella apicola sequence calculation
-
 6

General Information

EC Number General Information Comment Organism
1.6.2.4 additional information CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain Starmerella apicola