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Literature summary extracted from

  • Purchartova, K.; Engels, L.; Marhol, P.; Sulc, M.; Kuzma, M.; Slamova, K.; Elling, L.; Kren, V.
    Enzymatic preparation of silybin phase II metabolites: sulfation using aryl sulfotransferase from rat liver (2013), Appl. Microbiol. Biotechnol., 97, 10391-10398.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.1 adenosine 3',5'-bisphosphate
-
 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.1 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 33000
-
 x * 33000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + silybin B Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.8.2.1 Glycerol the addition of 10% glycerol (v/v) can double the storage stability of the enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Rattus norvegicus P17988
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 Ni2+NTA agarose column chromatography and Superdex 200 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 -20°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 4 days, 50% loss of activity Rattus norvegicus
2.8.2.1 37°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 6 h, complete loss of activity Rattus norvegicus
2.8.2.1 4°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 1 day, 50% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 r
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 r
2.8.2.1 3'-phosphoadenylyl sulfate + silybin B
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate
-
 ?
2.8.2.1 additional information silybin A is completely resistant to the sulfation reaction Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.1 ? x * 33000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 aryl sulfotransferase IV
-
 Rattus norvegicus
2.8.2.1 ASTIV
-
 Rattus norvegicus