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Literature summary extracted from

  • Durand, F.; Gounel, S.; Kjaergaard, C.H.; Solomon, E.I.; Mano, N.
    Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications (2012), Appl. Microbiol. Biotechnol., 96, 1489-1498.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.3.5 environmental protection the BOD from Magnaporthe oryzae is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications Pyricularia oryzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.5 overexpression of holo-enzyme in Pichia pastoris strain GS115, subcloning in Escherichia coli strain DH5alpha Pyricularia oryzae

General Stability

EC Number General Stability Organism
1.3.3.5 the recombinant enzyme shows high tolerance towards urea Pyricularia oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.5 NaCl 50% inhibition at about 10 mM, inactivation at 150 mM with substrate conjugated bilirubin Pyricularia oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ required, 4 ions per enzyme molecule Pyricularia oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.5 49000
-
 x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae
1.3.3.5 63975
-
 x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.5 2 bilirubin + O2 Pyricularia oryzae
-
 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2 Pyricularia oryzae ATCC MYA-4617
-
 2 biliverdin + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Pyricularia oryzae
-
-
-
1.3.3.5 Pyricularia oryzae ATCC MYA-4617
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5 recombinant holo-enzyme from Pichia pastoris strain GS115 to homogeneity by hydrophobic interaction chromatography Pyricularia oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2 bilirubin + O2
-
 Pyricularia oryzae 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2 substrate conjugated bilirubin Pyricularia oryzae 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2
-
 Pyricularia oryzae ATCC MYA-4617 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2 substrate conjugated bilirubin Pyricularia oryzae ATCC MYA-4617 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2
-
 Pyricularia oryzae ?
-
 ?
1.3.3.5 2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2
-
 Pyricularia oryzae ATCC MYA-4617 ?
-
 ?
1.3.3.5 additional information in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R Pyricularia oryzae ?
-
 ?
1.3.3.5 additional information in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R Pyricularia oryzae ATCC MYA-4617 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.5 ? x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 BOD
-
 Pyricularia oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.5 37
-
 assay at Pyricularia oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.5 37
-
 purified recombinant enzyme, half-life for deactivation is more than 300 min Pyricularia oryzae
1.3.3.5 60
-
 purified recombinant enzyme, half-life for deactivation is about 70 min Pyricularia oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 additional information
-
 the activity with laccase substrates 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine is assayed at pH 3.6 and pH 6.2, respectively Pyricularia oryzae
1.3.3.5 7.2
-
 assay at with bilirubin Pyricularia oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.3.5 1513
-
 bilirubin conjugated bilirubin, pH 7.0, 37°C Pyricularia oryzae