EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.3.5 | environmental protection | the BOD from Magnaporthe oryzae is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications | Pyricularia oryzae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.3.5 | overexpression of holo-enzyme in Pichia pastoris strain GS115, subcloning in Escherichia coli strain DH5alpha | Pyricularia oryzae |
EC Number | General Stability | Organism |
---|---|---|
1.3.3.5 | the recombinant enzyme shows high tolerance towards urea | Pyricularia oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.5 | NaCl | 50% inhibition at about 10 mM, inactivation at 150 mM with substrate conjugated bilirubin | Pyricularia oryzae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.5 | Cu2+ | required, 4 ions per enzyme molecule | Pyricularia oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 49000 | - |
x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination | Pyricularia oryzae |
1.3.3.5 | 63975 | - |
x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination | Pyricularia oryzae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.5 | 2 bilirubin + O2 | Pyricularia oryzae | - |
2 biliverdin + 2 H2O | - |
? | |
1.3.3.5 | 2 bilirubin + O2 | Pyricularia oryzae ATCC MYA-4617 | - |
2 biliverdin + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.5 | Pyricularia oryzae | - |
- |
- |
1.3.3.5 | Pyricularia oryzae ATCC MYA-4617 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.3.5 | recombinant holo-enzyme from Pichia pastoris strain GS115 to homogeneity by hydrophobic interaction chromatography | Pyricularia oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.5 | 2 bilirubin + O2 | - |
Pyricularia oryzae | 2 biliverdin + 2 H2O | - |
? | |
1.3.3.5 | 2 bilirubin + O2 | substrate conjugated bilirubin | Pyricularia oryzae | 2 biliverdin + 2 H2O | - |
? | |
1.3.3.5 | 2 bilirubin + O2 | - |
Pyricularia oryzae ATCC MYA-4617 | 2 biliverdin + 2 H2O | - |
? | |
1.3.3.5 | 2 bilirubin + O2 | substrate conjugated bilirubin | Pyricularia oryzae ATCC MYA-4617 | 2 biliverdin + 2 H2O | - |
? | |
1.3.3.5 | 2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2 | - |
Pyricularia oryzae | ? | - |
? | |
1.3.3.5 | 2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2 | - |
Pyricularia oryzae ATCC MYA-4617 | ? | - |
? | |
1.3.3.5 | additional information | in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R | Pyricularia oryzae | ? | - |
? | |
1.3.3.5 | additional information | in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R | Pyricularia oryzae ATCC MYA-4617 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.3.5 | ? | x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination | Pyricularia oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.3.5 | BOD | - |
Pyricularia oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 37 | - |
assay at | Pyricularia oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | 37 | - |
purified recombinant enzyme, half-life for deactivation is more than 300 min | Pyricularia oryzae |
1.3.3.5 | 60 | - |
purified recombinant enzyme, half-life for deactivation is about 70 min | Pyricularia oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.5 | additional information | - |
the activity with laccase substrates 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine is assayed at pH 3.6 and pH 6.2, respectively | Pyricularia oryzae |
1.3.3.5 | 7.2 | - |
assay at with bilirubin | Pyricularia oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.5 | 1513 | - |
bilirubin | conjugated bilirubin, pH 7.0, 37°C | Pyricularia oryzae |