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Literature summary extracted from

  • Winter, R.T.; Heuts, D.P.; Rijpkema, E.M.; van Bloois, E.; Wijma, H.J.; Fraaije, M.W.
    Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B (2012), Appl. Microbiol. Biotechnol., 95, 389-403.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.41 DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 Streptomyces coelicolor
1.1.3.41 gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Acidothermus cellulolyticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.41 E154P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 E50P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 K18R/E19R site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 L234R/D235P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 R232A/P233G site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 R232A/P233G/L234R/D235P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 T16R/A17P site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus
1.1.3.41 T16R/A17P/K18R/G19R site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme Acidothermus cellulolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.41 additional information
-
additional information steady-state kinetic analysis, overview Streptomyces coelicolor
1.1.3.41 additional information
-
additional information steady-state kinetic analysis, overview Acidothermus cellulolyticus
1.1.3.41 0.007
-
xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.32
-
xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.44
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 1.4
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 5.5
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 18
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 27
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 36
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 68
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 83
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 86
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 101
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 270
-
glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 350
-
glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.41 46800
-
about, sequence calculation Acidothermus cellulolyticus
1.1.3.41 47000
-
1 * 47000, SDS-PAGE Streptomyces coelicolor
1.1.3.41 47000
-
1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation Acidothermus cellulolyticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.41 Acidothermus cellulolyticus A0LST6
-
-
1.1.3.41 Acidothermus cellulolyticus 11B A0LST6
-
-
1.1.3.41 Streptomyces coelicolor Q9ZBU1
-
-
1.1.3.41 Streptomyces coelicolor A3(2) Q9ZBU1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.41 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, factor Xa protease cleavage of the His-tag Acidothermus cellulolyticus
1.1.3.41 recombinant His6-tagged enzyme from Escherichia coli strain MC1061 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus 11B ? + H2O2
-
?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor A3(2) ? + H2O2
-
?
1.1.3.41 (S)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 (S)-1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 (S)-1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus 11B ? + H2O2
-
?
1.1.3.41 (S)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor A3(2) ? + H2O2
-
?
1.1.3.41 1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 1-phenyl-1,2-ethanediol + O2
-
Acidothermus cellulolyticus 11B ? + H2O2
-
?
1.1.3.41 1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor A3(2) ? + H2O2
-
?
1.1.3.41 D-mannitol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 D-mannitol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 D-sorbitol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 D-sorbitol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 glycerol + O2
-
Streptomyces coelicolor ? + H2O2
-
?
1.1.3.41 glycerol + O2
-
Acidothermus cellulolyticus ? + H2O2
-
?
1.1.3.41 glycerol + O2
-
Acidothermus cellulolyticus 11B ? + H2O2
-
?
1.1.3.41 glycerol + O2
-
Streptomyces coelicolor A3(2) ? + H2O2
-
?
1.1.3.41 xylitol + O2
-
Streptomyces coelicolor xylose + H2O2
-
?
1.1.3.41 xylitol + O2
-
Acidothermus cellulolyticus xylose + H2O2
-
?
1.1.3.41 xylitol + O2
-
Acidothermus cellulolyticus 11B xylose + H2O2
-
?
1.1.3.41 xylitol + O2
-
Streptomyces coelicolor A3(2) xylose + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.41 monomer 1 * 47000, SDS-PAGE Streptomyces coelicolor
1.1.3.41 monomer 1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation Acidothermus cellulolyticus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.41 AldO
-
Streptomyces coelicolor
1.1.3.41 AldO
-
Acidothermus cellulolyticus
1.1.3.41 HotAldO
-
Streptomyces coelicolor
1.1.3.41 HotAldO
-
Acidothermus cellulolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.41 25
-
assay at Streptomyces coelicolor
1.1.3.41 25
-
assay at Acidothermus cellulolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.41 25 60 inactivation at 60°C and 50% remaining activity at 55°C Streptomyces coelicolor
1.1.3.41 25 84 AldO is a highly thermostable enzyme with an unfolding temperature of 84°C, inactivation above, and an activity half-life at 75°C of 112 min Acidothermus cellulolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.41 0.0004
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.008
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.1
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.1
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.36
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.74
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 1.2
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 1.3
-
glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 1.6
-
glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 1.9
-
xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 2.5
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 9.2
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 13
-
xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 17
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.41 7.5
-
assay at Streptomyces coelicolor
1.1.3.41 7.5
-
assay at Acidothermus cellulolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.41 FAD covalently bound, flavoenzyme Streptomyces coelicolor
1.1.3.41 FAD covalently bound, flavoenzyme Acidothermus cellulolyticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.41 0.00001
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.0001
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.0015
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.0043
-
1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.0046
-
glycerol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.0048
-
glycerol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.0056
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 0.0073
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.26
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 0.45
-
D-mannitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 2.7
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 12
-
D-sorbitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor
1.1.3.41 27
-
xylitol pH 7.5, 25°C, recombinant enzyme Acidothermus cellulolyticus
1.1.3.41 41
-
xylitol pH 7.5, 25°C, recombinant enzyme Streptomyces coelicolor