EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.41 | DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain MC1061 | Streptomyces coelicolor |
1.1.3.41 | gene aldO, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acidothermus cellulolyticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.41 | E154P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | E50P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | K18R/E19R | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | L234R/D235P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | R232A/P233G | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | R232A/P233G/L234R/D235P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | T16R/A17P | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
1.1.3.41 | T16R/A17P/K18R/G19R | site-directed mutagenesis, the mutant shows increased thermolability compared to the wild-type enzyme | Acidothermus cellulolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.41 | additional information | - |
additional information | steady-state kinetic analysis, overview | Streptomyces coelicolor | |
1.1.3.41 | additional information | - |
additional information | steady-state kinetic analysis, overview | Acidothermus cellulolyticus | |
1.1.3.41 | 0.007 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.32 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.44 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 1.4 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 5.5 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 18 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 27 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 36 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 68 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 83 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 86 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 101 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 270 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 350 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.41 | 46800 | - |
about, sequence calculation | Acidothermus cellulolyticus |
1.1.3.41 | 47000 | - |
1 * 47000, SDS-PAGE | Streptomyces coelicolor |
1.1.3.41 | 47000 | - |
1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation | Acidothermus cellulolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.41 | Acidothermus cellulolyticus | A0LST6 | - |
- |
1.1.3.41 | Acidothermus cellulolyticus 11B | A0LST6 | - |
- |
1.1.3.41 | Streptomyces coelicolor | Q9ZBU1 | - |
- |
1.1.3.41 | Streptomyces coelicolor A3(2) | Q9ZBU1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.41 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, factor Xa protease cleavage of the His-tag | Acidothermus cellulolyticus |
1.1.3.41 | recombinant His6-tagged enzyme from Escherichia coli strain MC1061 | Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.41 | (R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | (R)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | (R)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
1.1.3.41 | (R)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
1.1.3.41 | (S)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | (S)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | (S)-1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
1.1.3.41 | (S)-1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
1.1.3.41 | 1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | 1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | 1-phenyl-1,2-ethanediol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
1.1.3.41 | 1-phenyl-1,2-ethanediol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
1.1.3.41 | D-mannitol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | D-mannitol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | D-sorbitol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | D-sorbitol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | glycerol + O2 | - |
Streptomyces coelicolor | ? + H2O2 | - |
? | |
1.1.3.41 | glycerol + O2 | - |
Acidothermus cellulolyticus | ? + H2O2 | - |
? | |
1.1.3.41 | glycerol + O2 | - |
Acidothermus cellulolyticus 11B | ? + H2O2 | - |
? | |
1.1.3.41 | glycerol + O2 | - |
Streptomyces coelicolor A3(2) | ? + H2O2 | - |
? | |
1.1.3.41 | xylitol + O2 | - |
Streptomyces coelicolor | xylose + H2O2 | - |
? | |
1.1.3.41 | xylitol + O2 | - |
Acidothermus cellulolyticus | xylose + H2O2 | - |
? | |
1.1.3.41 | xylitol + O2 | - |
Acidothermus cellulolyticus 11B | xylose + H2O2 | - |
? | |
1.1.3.41 | xylitol + O2 | - |
Streptomyces coelicolor A3(2) | xylose + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.41 | monomer | 1 * 47000, SDS-PAGE | Streptomyces coelicolor |
1.1.3.41 | monomer | 1 * 47000, SDS-PAGE, 1 * 46800, about, sequence calculation | Acidothermus cellulolyticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.41 | AldO | - |
Streptomyces coelicolor |
1.1.3.41 | AldO | - |
Acidothermus cellulolyticus |
1.1.3.41 | HotAldO | - |
Streptomyces coelicolor |
1.1.3.41 | HotAldO | - |
Acidothermus cellulolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.41 | 25 | - |
assay at | Streptomyces coelicolor |
1.1.3.41 | 25 | - |
assay at | Acidothermus cellulolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.41 | 25 | 60 | inactivation at 60°C and 50% remaining activity at 55°C | Streptomyces coelicolor |
1.1.3.41 | 25 | 84 | AldO is a highly thermostable enzyme with an unfolding temperature of 84°C, inactivation above, and an activity half-life at 75°C of 112 min | Acidothermus cellulolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.41 | 0.0004 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.008 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.1 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.1 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.36 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.74 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 1.2 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 1.3 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 1.6 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 1.9 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 2.5 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 9.2 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 13 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 17 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.41 | 7.5 | - |
assay at | Streptomyces coelicolor |
1.1.3.41 | 7.5 | - |
assay at | Acidothermus cellulolyticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.41 | FAD | covalently bound, flavoenzyme | Streptomyces coelicolor | |
1.1.3.41 | FAD | covalently bound, flavoenzyme | Acidothermus cellulolyticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.41 | 0.00001 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.0001 | - |
(S)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.0015 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.0043 | - |
1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.0046 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.0048 | - |
glycerol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.0056 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 0.0073 | - |
(R)-1-phenyl-1,2-ethanediol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.26 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 0.45 | - |
D-mannitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 2.7 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 12 | - |
D-sorbitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor | |
1.1.3.41 | 27 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Acidothermus cellulolyticus | |
1.1.3.41 | 41 | - |
xylitol | pH 7.5, 25°C, recombinant enzyme | Streptomyces coelicolor |