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Literature summary extracted from

  • Araya-Garay, J.M.; Feijoo-Siota, L.; Rosa-dos-Santos, F.; Veiga-Crespo, P.; Villa, T.G.
    Construction of new Pichia pastoris X-33 strains for production of lycopene and beta-carotene (2012), Appl. Microbiol. Biotechnol., 93, 2483-2492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 gene crtI, recombinant coexpression of genes crtE, crtB, and crtI from Erwinia uredovora and of crtL from Ficus carica in Pichia pastoris X-33 strains for large-scale biosynthesis of carotenoids, subcloning of crtI in Escherichhia coli strain TOP10 Pantoea ananatis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.31 additional information enzyme modification by site-directed mutagenesis, overview Pantoea ananatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + acceptor Pantoea ananatis
-
 all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor Pantoea ananatis
-
 all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + acceptor Pantoea ananatis
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + acceptor Pantoea ananatis
-
 all-trans-neurosporene + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Pantoea ananatis P21685 gene crtI
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + acceptor
-
 Pantoea ananatis all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor
-
 Pantoea ananatis all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + acceptor
-
 Pantoea ananatis all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + acceptor
-
 Pantoea ananatis all-trans-neurosporene + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.31 CrtI
-
 Pantoea ananatis
1.3.99.31 phytoene desaturase
-
 Pantoea ananatis

General Information

EC Number General Information Comment Organism
1.3.99.31 metabolism the enzyme is involved catalyzing several steps in the beta-carotene biosynthetic pathway via phytoene, zeta-carotene, and lycopene, overview Pantoea ananatis