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Literature summary extracted from

  • Kolvenbach, B.A.; Dobrowinski, H.; Fousek, J.; Vlcek, C.; Schaeffer, A.; Gabriel, F.L.; Kohler, H.P.; Corvini, P.F.
    An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp. strain TTNP3 (2012), Appl. Microbiol. Biotechnol., 93, 1315-1324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.61 HqdC expression in Escherichia coli BL21-DE3 cells Sphingomonas sp.
1.3.1.32 expressed in Escherichia coli Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.61 53500
-
 calculated from sequence Sphingomonas sp.
1.3.1.32 37100
-
 SDS-PAGE Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.61 Sphingomonas sp. F8TW85
-
-
1.2.1.61 Sphingomonas sp. TTNP3 F8TW85
-
-
1.3.1.32 Sphingomonas sp.
-
-
-
1.3.1.32 Sphingomonas sp. TTNP3
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.61 0.0006
-
 uninduced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.
1.2.1.61 0.0025
-
 hydroquinone-induced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.
1.2.1.61 0.0031
-
 nonylphenol-induced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Sphingomonas sp. maleylacetate + NADH + 2 H+
-
 ?
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Sphingomonas sp. TTNP3 maleylacetate + NADH + 2 H+
-
 ?
1.3.1.32 2-maleylacetate + NADPH
-
 Sphingomonas sp. 3-oxoadipate + NADP+
-
 ?
1.3.1.32 2-maleylacetate + NADPH
-
 Sphingomonas sp. TTNP3 3-oxoadipate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.61 4-hydroxymuconic semialdehyde dehydrogenase
-
 Sphingomonas sp.
1.2.1.61 HqdC
-
 Sphingomonas sp.
1.3.1.32 hqdD
-
 Sphingomonas sp.
1.3.1.32 maleylacetate reductase
-
 Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7
-
 assay at Sphingomonas sp.

Expression

EC Number Organism Comment Expression
1.2.1.61 Sphingomonas sp. by 0.5 mM hydroquinone and by 0.5 mM nonylphenol up

General Information

EC Number General Information Comment Organism
1.2.1.61 metabolism HqdC gene is part of a gene cluster associated with hydroquinone degradation into 3-oxoadipate Sphingomonas sp.