EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.B2 | expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells | Pyrobaculum calidifontis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.B2 | 0.026 | - |
2,6-dichlorophenolindophenol | with L-proline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50°C | Pyrobaculum calidifontis | |
1.5.99.B2 | 0.035 | - |
2,6-dichlorophenolindophenol | with L-hydroxyproline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50°C | Pyrobaculum calidifontis | |
1.5.99.B2 | 1.67 | - |
L-proline | in 200 mM Tris-HCl buffer (pH 8.0), at 50°C | Pyrobaculum calidifontis | |
1.5.99.B2 | 6.05 | - |
L-hydroxyproline | in 200 mM Tris-HCl buffer (pH 8.0), at 50°C | Pyrobaculum calidifontis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 46000 | - |
2 * 46000, SDS-PAGE | Pyrobaculum calidifontis |
1.5.99.B2 | 46259 | - |
2 * 46259, calculated from amino acid sequence | Pyrobaculum calidifontis |
1.5.99.B2 | 108000 | - |
gel filtration | Pyrobaculum calidifontis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.B2 | Pyrobaculum calidifontis | - |
- |
- |
1.5.99.B2 | Pyrobaculum calidifontis JCM 11548 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.B2 | DEAE Toyopearl 650M column chromatography and Superdex 200 gel filtration | Pyrobaculum calidifontis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 0.026 | - |
crude extract, pH 8.0, at 50°C | Pyrobaculum calidifontis |
1.5.99.B2 | 4.25 | - |
after 163fold purification, pH 8.0, at 50°C | Pyrobaculum calidifontis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.B2 | L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O | 72% activity compared to L-proline | Pyrobaculum calidifontis | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O | 72% activity compared to L-proline | Pyrobaculum calidifontis JCM 11548 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-proline + 2,6-dichlorophenolindophenol + H2O | L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor | Pyrobaculum calidifontis | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-proline + 2,6-dichlorophenolindophenol + H2O | L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor | Pyrobaculum calidifontis JCM 11548 | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-proline + 4-iodonitrotetrazolium violet + H2O | 9% activity compared to 2,6-dichlorophenolindophenol | Pyrobaculum calidifontis | DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet | - |
? | |
1.5.99.B2 | L-proline + 4-iodonitrotetrazolium violet + H2O | 9% activity compared to 2,6-dichlorophenolindophenol | Pyrobaculum calidifontis JCM 11548 | DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet | - |
? | |
1.5.99.B2 | L-proline + ferricyanide | 93% activity compared to 2,6-dichlorophenolindophenol | Pyrobaculum calidifontis | DELTA1-pyrroline-5-carboxylate + ferrocyanide | - |
? | |
1.5.99.B2 | L-proline + ferricyanide | 93% activity compared to 2,6-dichlorophenolindophenol | Pyrobaculum calidifontis JCM 11548 | DELTA1-pyrroline-5-carboxylate + ferrocyanide | - |
? | |
1.5.99.B2 | additional information | sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors | Pyrobaculum calidifontis | ? | - |
? | |
1.5.99.B2 | additional information | sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors | Pyrobaculum calidifontis JCM 11548 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.99.B2 | homodimer | 2 * 46000, SDS-PAGE | Pyrobaculum calidifontis |
1.5.99.B2 | homodimer | 2 * 46259, calculated from amino acid sequence | Pyrobaculum calidifontis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.B2 | L-proDH | - |
Pyrobaculum calidifontis |
1.5.99.B2 | L-proline dehydrogenase | - |
Pyrobaculum calidifontis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 80 | - |
the optimum temperature for enzyme activity is above 80°C | Pyrobaculum calidifontis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 100 | 105 | the enzyme retains more than 90% of its activity after incubation at 100°C for 120 min (pH 7.5). The half-life at 105°C is about 90 min | Pyrobaculum calidifontis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 7 | - |
- |
Pyrobaculum calidifontis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 6 | 9 | half of the activity is retained at pH 6.0 and 9.0 | Pyrobaculum calidifontis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 4.5 | 9 | the enzyme retains more than 90% of its activity after incubation at pH values 4.5-9.0 for 30 min at 50°C | Pyrobaculum calidifontis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.B2 | FAD | - |
Pyrobaculum calidifontis |