EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.2.8 | gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
1.1.2.8 | gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
1.1.2.8 | gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.8 | ethanol + 2 ferricytochrome c | Acetobacter pasteurianus | - |
ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | Acetobacter pasteurianus MSU10 | - |
ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | Acetobacter pasteurianus IFO3191 | - |
ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | Acetobacter pasteurianus SKU1108 | - |
ethanal + 2 ferrocytochrome c | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Acetobacter pasteurianus | - |
acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Acetobacter pasteurianus MSU10 | - |
acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Acetobacter pasteurianus IFO3191 | - |
acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Acetobacter pasteurianus SKU1108 | - |
acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Acetobacter pasteurianus IFO3284 | - |
acetaldehyde + ubiquinol | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.2.8 | acetic acid | ADH from strain IFO3191 retains 36% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 45% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
1.1.2.8 | acetic acid | ADH from strain MSU10 shows the highest resistance to acetic acid. The enzyme retains over 90% of the original activity after 30 min incubation with 2% acetic acid at 30°C and also with 4% acetic acid at 25°C, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
1.1.2.8 | acetic acid | ADH from strain SKU1108 retains 61% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 79% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
1.1.5.5 | Ethanol | ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature | Acetobacter pasteurianus |
1.1.5.5 | Ethanol | ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain IFO3191 loses all the activity at 40°C at 22% ethanol | Acetobacter pasteurianus |
1.1.5.5 | Ethanol | ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain MSU10 shows 43% remaining activity at 40°C at 22% ethanol | Acetobacter pasteurianus |
1.1.5.5 | Ethanol | ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain SKU1108 shows 29% reamining activity at 40°C at 22% ethanol | Acetobacter pasteurianus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.8 | Acetobacter pasteurianus | C7G3B7 | gene adhA encoding subunit I; isolated from passion fruit | - |
1.1.2.8 | Acetobacter pasteurianus | C7G3B8 | gene adhB encoding subunit II; isolated from passion fruit | - |
1.1.2.8 | Acetobacter pasteurianus | C9K501 | gene adhA encoding subunit I | - |
1.1.2.8 | Acetobacter pasteurianus | C9K502 | gene adhB encoding subunit II | - |
1.1.2.8 | Acetobacter pasteurianus | C9K504 | gene adhS encoding subunit III | - |
1.1.2.8 | Acetobacter pasteurianus | C9W9E3 | gene adhS encoding the subunit III | - |
1.1.2.8 | Acetobacter pasteurianus | D2SZY4 | gene adhA encoding the large subunit | - |
1.1.2.8 | Acetobacter pasteurianus | D2SZY5 | gene adhB encoding the cytochrome c subunit | - |
1.1.2.8 | Acetobacter pasteurianus IFO3191 | C9K501 | gene adhA encoding subunit I | - |
1.1.2.8 | Acetobacter pasteurianus IFO3191 | C9K502 | gene adhB encoding subunit II | - |
1.1.2.8 | Acetobacter pasteurianus IFO3191 | C9K504 | gene adhS encoding subunit III | - |
1.1.2.8 | Acetobacter pasteurianus MSU10 | C7G3B7 | gene adhA encoding subunit I; isolated from passion fruit | - |
1.1.2.8 | Acetobacter pasteurianus MSU10 | C7G3B8 | gene adhB encoding subunit II; isolated from passion fruit | - |
1.1.2.8 | Acetobacter pasteurianus SKU1108 | C9W9E3 | gene adhS encoding the subunit III | - |
1.1.2.8 | Acetobacter pasteurianus SKU1108 | D2SZY4 | gene adhA encoding the large subunit | - |
1.1.2.8 | Acetobacter pasteurianus SKU1108 | D2SZY5 | gene adhB encoding the cytochrome c subunit | - |
1.1.5.5 | Acetobacter pasteurianus | - |
- |
- |
1.1.5.5 | Acetobacter pasteurianus | - |
subsp lovaniensis | - |
1.1.5.5 | Acetobacter pasteurianus IFO3191 | - |
- |
- |
1.1.5.5 | Acetobacter pasteurianus IFO3284 | - |
subsp lovaniensis | - |
1.1.5.5 | Acetobacter pasteurianus MSU10 | - |
- |
- |
1.1.5.5 | Acetobacter pasteurianus SKU1108 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.2.8 | native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
1.1.2.8 | native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
1.1.2.8 | native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.2.8 | additional information | the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while strain SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strain IFO3191 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol | Acetobacter pasteurianus | - |
1.1.5.5 | cell culture | the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while the SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strains IFO3191 and IFO3284 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol | Acetobacter pasteurianus | - |
1.1.5.5 | additional information | growth and tolerance to acetic acid and ethanol of thermotolerant strains at several conditions, overview | Acetobacter pasteurianus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.8 | ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus | ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus MSU10 | ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus IFO3191 | ethanal + 2 ferrocytochrome c | - |
? | |
1.1.2.8 | ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus SKU1108 | ethanal + 2 ferrocytochrome c | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Acetobacter pasteurianus | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Acetobacter pasteurianus MSU10 | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Acetobacter pasteurianus IFO3191 | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Acetobacter pasteurianus SKU1108 | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Acetobacter pasteurianus IFO3284 | acetaldehyde + ubiquinol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.8 | quinoprotein alcohol dehydrogenase | - |
Acetobacter pasteurianus |
1.1.5.5 | quinoprotein alcohol dehydrogenase | - |
Acetobacter pasteurianus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.2.8 | 30 | - |
- |
Acetobacter pasteurianus |
1.1.2.8 | 35 | - |
- |
Acetobacter pasteurianus |
1.1.5.5 | 25 | - |
assay at | Acetobacter pasteurianus |
1.1.5.5 | 30 | - |
- |
Acetobacter pasteurianus |
1.1.5.5 | 35 | - |
- |
Acetobacter pasteurianus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | additional information | - |
ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature | Acetobacter pasteurianus |
1.1.5.5 | 45 | - |
30 min, stable up to | Acetobacter pasteurianus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.8 | 6 | - |
assay at | Acetobacter pasteurianus |
1.1.5.5 | 6 | - |
assay at | Acetobacter pasteurianus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.8 | pyrroloquinoline quinone | - |
Acetobacter pasteurianus | |
1.1.5.5 | pyrroloquinoline quinone | dependent on | Acetobacter pasteurianus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.2.8 | physiological function | the ADH involved in ethanol oxidation of the thermotolerant strain is important for the high temperature fermentation | Acetobacter pasteurianus |