Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kanchanarach, W.; Theeragool, G.; Yakushi, T.; Toyama, H.; Adachi, O.; Matsushita, K.
    Characterization of thermotolerant Acetobacter pasteurianus strains and their quinoprotein alcohol dehydrogenases (2010), Appl. Microbiol. Biotechnol., 85, 741-751.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.8 gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus
1.1.2.8 gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus
1.1.2.8 gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.8 ethanol + 2 ferricytochrome c Acetobacter pasteurianus
-
ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c Acetobacter pasteurianus MSU10
-
ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c Acetobacter pasteurianus IFO3191
-
ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c Acetobacter pasteurianus SKU1108
-
ethanal + 2 ferrocytochrome c
-
?
1.1.5.5 ethanol + ubiquinone Acetobacter pasteurianus
-
acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone Acetobacter pasteurianus MSU10
-
acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone Acetobacter pasteurianus IFO3191
-
acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone Acetobacter pasteurianus SKU1108
-
acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone Acetobacter pasteurianus IFO3284
-
acetaldehyde + ubiquinol
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.2.8 acetic acid ADH from strain IFO3191 retains 36% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 45% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C Acetobacter pasteurianus
1.1.2.8 acetic acid ADH from strain MSU10 shows the highest resistance to acetic acid. The enzyme retains over 90% of the original activity after 30 min incubation with 2% acetic acid at 30°C and also with 4% acetic acid at 25°C, unstable in 4% acetic acid at 30°C Acetobacter pasteurianus
1.1.2.8 acetic acid ADH from strain SKU1108 retains 61% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 79% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C Acetobacter pasteurianus
1.1.5.5 Ethanol ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature Acetobacter pasteurianus
1.1.5.5 Ethanol ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain IFO3191 loses all the activity at 40°C at 22% ethanol Acetobacter pasteurianus
1.1.5.5 Ethanol ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain MSU10 shows 43% remaining activity at 40°C at 22% ethanol Acetobacter pasteurianus
1.1.5.5 Ethanol ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature, the ADH from strain SKU1108 shows 29% reamining activity at 40°C at 22% ethanol Acetobacter pasteurianus

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.8 Acetobacter pasteurianus C7G3B7 gene adhA encoding subunit I; isolated from passion fruit
-
1.1.2.8 Acetobacter pasteurianus C7G3B8 gene adhB encoding subunit II; isolated from passion fruit
-
1.1.2.8 Acetobacter pasteurianus C9K501 gene adhA encoding subunit I
-
1.1.2.8 Acetobacter pasteurianus C9K502 gene adhB encoding subunit II
-
1.1.2.8 Acetobacter pasteurianus C9K504 gene adhS encoding subunit III
-
1.1.2.8 Acetobacter pasteurianus C9W9E3 gene adhS encoding the subunit III
-
1.1.2.8 Acetobacter pasteurianus D2SZY4 gene adhA encoding the large subunit
-
1.1.2.8 Acetobacter pasteurianus D2SZY5 gene adhB encoding the cytochrome c subunit
-
1.1.2.8 Acetobacter pasteurianus IFO3191 C9K501 gene adhA encoding subunit I
-
1.1.2.8 Acetobacter pasteurianus IFO3191 C9K502 gene adhB encoding subunit II
-
1.1.2.8 Acetobacter pasteurianus IFO3191 C9K504 gene adhS encoding subunit III
-
1.1.2.8 Acetobacter pasteurianus MSU10 C7G3B7 gene adhA encoding subunit I; isolated from passion fruit
-
1.1.2.8 Acetobacter pasteurianus MSU10 C7G3B8 gene adhB encoding subunit II; isolated from passion fruit
-
1.1.2.8 Acetobacter pasteurianus SKU1108 C9W9E3 gene adhS encoding the subunit III
-
1.1.2.8 Acetobacter pasteurianus SKU1108 D2SZY4 gene adhA encoding the large subunit
-
1.1.2.8 Acetobacter pasteurianus SKU1108 D2SZY5 gene adhB encoding the cytochrome c subunit
-
1.1.5.5 Acetobacter pasteurianus
-
-
-
1.1.5.5 Acetobacter pasteurianus
-
subsp lovaniensis
-
1.1.5.5 Acetobacter pasteurianus IFO3191
-
-
-
1.1.5.5 Acetobacter pasteurianus IFO3284
-
subsp lovaniensis
-
1.1.5.5 Acetobacter pasteurianus MSU10
-
-
-
1.1.5.5 Acetobacter pasteurianus SKU1108
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.8 native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus
1.1.2.8 native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus
1.1.2.8 native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.2.8 additional information the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while strain SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strain IFO3191 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol Acetobacter pasteurianus
-
1.1.5.5 cell culture the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while the SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strains IFO3191 and IFO3284 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol Acetobacter pasteurianus
-
1.1.5.5 additional information growth and tolerance to acetic acid and ethanol of thermotolerant strains at several conditions, overview Acetobacter pasteurianus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.8 ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus MSU10 ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus IFO3191 ethanal + 2 ferrocytochrome c
-
?
1.1.2.8 ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus SKU1108 ethanal + 2 ferrocytochrome c
-
?
1.1.5.5 ethanol + ubiquinone
-
Acetobacter pasteurianus acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone
-
Acetobacter pasteurianus MSU10 acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone
-
Acetobacter pasteurianus IFO3191 acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone
-
Acetobacter pasteurianus SKU1108 acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone
-
Acetobacter pasteurianus IFO3284 acetaldehyde + ubiquinol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.8 quinoprotein alcohol dehydrogenase
-
Acetobacter pasteurianus
1.1.5.5 quinoprotein alcohol dehydrogenase
-
Acetobacter pasteurianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.8 30
-
-
Acetobacter pasteurianus
1.1.2.8 35
-
-
Acetobacter pasteurianus
1.1.5.5 25
-
assay at Acetobacter pasteurianus
1.1.5.5 30
-
-
Acetobacter pasteurianus
1.1.5.5 35
-
-
Acetobacter pasteurianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.5 additional information
-
ADHs from MSU10 and SKU1108 strains exhibit a higher resistance to ethanol and acetic acid than strain IFO3191 enzyme at elevated temperature Acetobacter pasteurianus
1.1.5.5 45
-
30 min, stable up to Acetobacter pasteurianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.8 6
-
assay at Acetobacter pasteurianus
1.1.5.5 6
-
assay at Acetobacter pasteurianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.8 pyrroloquinoline quinone
-
Acetobacter pasteurianus
1.1.5.5 pyrroloquinoline quinone dependent on Acetobacter pasteurianus

General Information

EC Number General Information Comment Organism
1.1.2.8 physiological function the ADH involved in ethanol oxidation of the thermotolerant strain is important for the high temperature fermentation Acetobacter pasteurianus