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Literature summary extracted from

  • Salvachua, D.; Prieto, A.; Martinez, A.T.; Martinez, M.J.
    Characterization of a novel dye-decolorizing peroxidase (DyP)-type enzyme from Irpex lacteus and its application in enzymatic hydrolysis of wheat straw (2013), Appl. Environ. Microbiol., 79, 4316-4324.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.19 H2O2 maximum relative activity at final H2O2 concentrations between 0.4 and 0.8 mM, activity then gradually drops off. A residual activity of 30% still remains at 12.5 mM H2O2, and the enzyme is completely inactivated in the presence of 50 mM H2O2 Irpex lacteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.0112
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 0.0135
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 0.028
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3,0, temperature not specified in the publication Irpex lacteus
1.11.1.19 0.0726
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 0.0795
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 3.61
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Irpex lacteus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.19 37.8
-
 substrate 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate, pH 5.0, temperature not specified in the publication Irpex lacteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2 2,6-dimethoxyphenol + 2 H2O2
-
 Irpex lacteus coerulignone + 2 H2O
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Irpex lacteus ? + H2O
-
 ?
1.11.1.19 Reactive Black 5 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Black 5 + H2O
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 19 + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+
-
 Irpex lacteus veratraldehyde + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 2.7
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 11.9
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 70.9
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 79.9
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 224
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3,0, temperature not specified in the publication Irpex lacteus
1.11.1.19 419
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.83
-
 veratryl alcohol pH 2.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 970
-
 2,6-dimethoxyphenol pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 1100
-
 Reactive Black 5 pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 5300
-
 H2O2 pH 3.0, temperature not specified in the publication Irpex lacteus
1.11.1.19 5900
-
 Reactive Blue 19 pH 4.0, temperature not specified in the publication Irpex lacteus