EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.4 | gene nox, DNA and amino acid sequence deteremination and analysis, recombinant expression from a broad-host-range cloning vector in Escherichia coli strain DH5alpha and Pseudomonas putida strain KT2440, the enzyme is exxpressed in both hosts but only active in Pseudomonas putida | Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.99.4 | H456R | site-directed mutagenesis | Pseudomonas sp. |
1.5.99.4 | additional information | construction of the nox deletion mutant strain, that loses the ability to degrade nicotine, but not pseudooxynicotine | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.4 | (S)-nicotine + acceptor + H2O | Pseudomonas sp. | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | pseudooxynicotine + reduced acceptor | - |
? | |
1.5.99.4 | (S)-nicotine + acceptor + H2O | Pseudomonas sp. HZN6 | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | pseudooxynicotine + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.4 | no activity in Escherichia coli strain DH5alpha | - |
- |
- |
1.5.99.4 | no activity in Pseudomonas putida strain KT2440 | - |
- |
- |
1.5.99.4 | Pseudomonas sp. | M4T5L3 | gene nox | - |
1.5.99.4 | Pseudomonas sp. HZN6 | M4T5L3 | gene nox | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.4 | (RS)-nicotine + acceptor + H2O | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | Pseudomonas sp. | pseudooxynicotine + reduced acceptor | - |
? | |
1.5.99.4 | (RS)-nicotine + acceptor + H2O | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | Pseudomonas sp. HZN6 | pseudooxynicotine + reduced acceptor | - |
? | |
1.5.99.4 | (S)-nicotine + acceptor + H2O | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | Pseudomonas sp. | pseudooxynicotine + reduced acceptor | - |
? | |
1.5.99.4 | (S)-nicotine + acceptor + H2O | via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity | Pseudomonas sp. HZN6 | pseudooxynicotine + reduced acceptor | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.4 | FAD | conserved FAD-binding GXGXXG | Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.99.4 | malfunction | a nox disruption mutant of strain HZN6 loses the ability to degrade nicotine, but not pseudooxynicotine | Pseudomonas sp. |
1.5.99.4 | metabolism | the enzyme is responsible for the first step of nicotine degradation | Pseudomonas sp. |
1.5.99.4 | additional information | the conserved FAD-binding GXGXXG motif and His456 are essential for nicotine degradation activity | Pseudomonas sp. |
1.5.99.4 | physiological function | the enzyme nonenantioselectively degrades nicotine to pseudooxynicotine | Pseudomonas sp. |