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Literature summary extracted from

  • Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.
    Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (2013), Appl. Environ. Microbiol., 79, 113-120.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.2.2 sulfite 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM Acidithiobacillus ferrooxidans
1.8.5.2 Sodium sulfate the purified enzyme requires 150 mM sodium sulfate to generate maximum activity Acidithiobacillus ferrooxidans
1.8.5.2 sulfite the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold Acidithiobacillus ferrooxidans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.2 expression in Escherichia coli Acidithiobacillus ferrooxidans
1.8.5.2 expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.2 sulfate 200 mM, about 60% reduction in activity Acidithiobacillus ferrooxidans
1.8.2.2 sulfite 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM Acidithiobacillus ferrooxidans
1.8.5.2 Sodium sulfate 60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate Acidithiobacillus ferrooxidans
1.8.5.2 sulfite almost complete inhibition in the presence of 10 mM sulfite Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 8
-
 thiosulfate recombinant protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans
1.8.2.2 15
-
 thiosulfate native protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans
1.8.5.2 8
-
 thiosulfate recombinant enzyme, at pH 2.5 and 50°C Acidithiobacillus ferrooxidans
1.8.5.2 15
-
 thiosulfate native enzyme, at pH 2.5 and 70°C Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.2 25000
-
 x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans
1.8.2.2 25796
-
 x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans
1.8.5.2 25000
-
 x * 25000, SDS-PAGE Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Acidithiobacillus ferrooxidans B7J3E3
-
-
1.8.5.2 Acidithiobacillus ferrooxidans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.2.2 proteolytic modification sequence contains a signal peptide of 37 amino acids Acidithiobacillus ferrooxidans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 partial Acidithiobacillus ferrooxidans
1.8.5.2 ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration Acidithiobacillus ferrooxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.2 culture condition:sulfur-grown cell
-
 Acidithiobacillus ferrooxidans
-
1.8.5.2 culture condition:tetrathionate-grown cell
-
 Acidithiobacillus ferrooxidans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 4.16
-
 pH 2.5, 40°C, recombinant protein Acidithiobacillus ferrooxidans
1.8.2.2 6.5
-
 native protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans
1.8.5.2 0.05
-
 unpurified recombinant enzyme, at pH 2.5 and 40°C Acidithiobacillus ferrooxidans
1.8.5.2 3.16
-
 recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 2 thiosulfate + 2 ferricyanide no catalytic activity below 4 mM thiosulfate. Enzyme catalyzes the formation of 1 mol of tetrathionate from 2 mol of thiosulfate Acidithiobacillus ferrooxidans tetrathionate + 2 ferrocyanide
-
 ?
1.8.2.2 additional information no substrates: sulfite, ubiquinone and horse heart cytochrome c Acidithiobacillus ferrooxidans ?
-
 ?
1.8.5.2 additional information the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor Acidithiobacillus ferrooxidans ?
-
 ?
1.8.5.2 thiosulfate + ferricyanide + H+ when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected Acidithiobacillus ferrooxidans tetrathionate + ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.2 ? x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans
1.8.5.2 ? x * 25000, SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.2.2 AFE_0042 locus name Acidithiobacillus ferrooxidans
1.8.2.2 Tat pathway signal sequence domain protein
-
 Acidithiobacillus ferrooxidans
1.8.5.2 tetrathionate-forming thiosulfate dehydrogenase
-
 Acidithiobacillus ferrooxidans
1.8.5.2 TSD
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.2 50
-
 recombinant protein Acidithiobacillus ferrooxidans
1.8.2.2 70
-
 native protein Acidithiobacillus ferrooxidans
1.8.5.2 50
-
 recombinant enzyme Acidithiobacillus ferrooxidans
1.8.5.2 70
-
 native enzyme Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 2.5
-
-
 Acidithiobacillus ferrooxidans
1.8.5.2 2.5
-
 native and recombinant enzyme Acidithiobacillus ferrooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.5.2 2 3 the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.2 additional information no cofactor: heme Acidithiobacillus ferrooxidans
1.8.5.2 additional information does not contain heme Acidithiobacillus ferrooxidans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.2.2 Acidithiobacillus ferrooxidans calculated, mature protein
-
 6.2

General Information

EC Number General Information Comment Organism
1.8.2.2 metabolism enzyme activity is detected in strains grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells Acidithiobacillus ferrooxidans