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Literature summary extracted from

  • Uthoff, S.; Steinbuechel, A.
    Purification and characterization of an NAD+-dependent XylB-like aryl alcohol dehydrogenase identified in Acinetobacter baylyi ADP1 (2012), Appl. Environ. Microbiol., 78, 8743-8752.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.90 expressed in Escherichia coli as a His-tagged fusion protein Acinetobacter baylyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 1,10-phenanthroline weak inhibition Acinetobacter baylyi
1.1.1.90 N-ethylmaleimide
-
 Acinetobacter baylyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.017
-
 NADH pH 7.4, 30°C Acinetobacter baylyi
1.1.1.90 0.28
-
 benzaldehyde pH 7.4, 30°C Acinetobacter baylyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.90 Ag+ 0.01 mM, residual activity: 0.2% Acinetobacter baylyi
1.1.1.90 Cu2+ 0.1 mM, residual activity: 1.3% Acinetobacter baylyi
1.1.1.90 Fe2+ 1 mM, residual activity: 21.7% Acinetobacter baylyi
1.1.1.90 Hg+ 0.001 mM, residual activity: 0.1% Acinetobacter baylyi
1.1.1.90 Mg2+ 1 mM, activity enhanced to 128% Acinetobacter baylyi
1.1.1.90 Mn2+ 1 mM, activity enhanced to 128% Acinetobacter baylyi
1.1.1.90 Zn2+ 1 mM, residual activity: 1.2% Acinetobacter baylyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 39500
-
 SDS-PAGE Acinetobacter baylyi
1.1.1.90 72600
-
 gel filtration Acinetobacter baylyi

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Acinetobacter baylyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90 ion-exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, affinity chromatography Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 anisaldehyde + NADH + H+ relative activity: 72.2% Acinetobacter baylyi anisic alcohol + NAD+
-
 r
1.1.1.90 benzaldehyde + NADH + H+ relative activity: 100% Acinetobacter baylyi benzyl alcohol + NAD+
-
 r
1.1.1.90 cinnamaldehyde + NADH + H+ relative activity: 58.7% Acinetobacter baylyi cinnamic alcohol + NAD+
-
 r
1.1.1.90 cis-11-hexadecenal + NADH + H+ relative activity: 4.9% Acinetobacter baylyi cis-11-hexadecenol + NAD+
-
 r
1.1.1.90 citral + NADH + H+ relative activity: 7.8% Acinetobacter baylyi citryl alcohol + NAD+
-
 r
1.1.1.90 citronellal + NADH + H+ relative activity: 21.8% Acinetobacter baylyi citronellol + NAD+
-
 r
1.1.1.90 coniferyl alcohol + NAD+ relative activity: 96% Acinetobacter baylyi coniferyl aldehyde + NADH + H+
-
 r
1.1.1.90 decanal + NADH + H+ relative activity: 19.7% Acinetobacter baylyi decanol + NAD+
-
 r
1.1.1.90 dodecanal + NADH + H+ relative activity: 8.9% Acinetobacter baylyi dodecanol + NAD+
-
 r
1.1.1.90 geranial + NADH + H+
-
 Acinetobacter baylyi geraniol + NAD+
-
 r
1.1.1.90 glutaraldehyde + NADH + H+ relative activity: 0.6% Acinetobacter baylyi ? + NAD+
-
 r
1.1.1.90 glyoxal + NADH + H+ relative activity: 0.7% Acinetobacter baylyi ? + NAD+
-
 r
1.1.1.90 hexanal + NADH + H+ relative activity: 6.4% Acinetobacter baylyi hexanol + NAD+
-
 r
1.1.1.90 octanal + NADH + H+ relative activity: 36.5% Acinetobacter baylyi octanol + NAD+
-
 r
1.1.1.90 salicyl aldehyde + NADH + H+ relative activity: 9% Acinetobacter baylyi salicyl alcohol + NAD+
-
 r
1.1.1.90 vanillin + NADH + H+ relative activity: 4% Acinetobacter baylyi vanillin alcohol + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.90 dimer 2 * 39500, gel filtration Acinetobacter baylyi

Synonyms

EC Number Synonyms Comment Organism
1.1.1.90 ADP1
-
 Acinetobacter baylyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.90 40
-
-
 Acinetobacter baylyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.90 15
-
 enzyme reaches 52% of its maximum activity at 15°C Acinetobacter baylyi
1.1.1.90 70
-
 enzyme loses 94% of its activity after 2 min of incubation at 70°C Acinetobacter baylyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 8920
-
 NADH pH 7.4, 30°C Acinetobacter baylyi
1.1.1.90 12940
-
 benzaldehyde pH 7.4, 30°C Acinetobacter baylyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 6.5
-
-
 Acinetobacter baylyi

General Information

EC Number General Information Comment Organism
1.1.1.90 malfunction disruption mutant shows similar growth compared to wild-type Acinetobacter baylyi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.90 46700
-
 benzaldehyde pH 7.4, 30°C Acinetobacter baylyi
1.1.1.90 528000
-
 NADH pH 7.4, 30°C Acinetobacter baylyi