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Literature summary extracted from

  • Sygmund, C.; Kracher, D.; Scheiblbrandner, S.; Zahma, K.; Felice, A.K.; Harreither, W.; Kittl, R.; Ludwig, R.
    Characterization of the two Neurospora crassa cellobiose dehydrogenases and their connection to oxidative cellulose degradation (2012), Appl. Environ. Microbiol., 78, 6161-6171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expressed in Pichia pastoris strain X-33 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.022
-
 cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.043
-
 cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.045
-
 cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.061
-
 cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.089
-
 lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.196
-
 cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.204
-
 cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.23
-
 cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.29
-
 lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.81
-
 xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.9
-
 cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.2
-
 maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.31
-
 xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3
-
 xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.44
-
 maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.6
-
 xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 4.5
-
 maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 8.4
-
 Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 17.2
-
 maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 34.3
-
 maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 35.7
-
 maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 39.2
-
 Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 550
-
 D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1545
-
 D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2770
-
 D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3600
-
 D-galactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3700
-
 D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 10000
-
 D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 13300
-
 D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 85000
-
 x * 85000, isoform CDH IIA, SDS-PAGE Neurospora crassa
1.1.99.18 85800
-
 x * 85800, isoform CDH IIB, SDS-PAGE Neurospora crassa
1.1.99.18 110000 130000 gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 1,4-benzoquinone
-
 Neurospora crassa cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Neurospora crassa cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellopentaose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellotetraose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellotriose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellulose + ferrocenium hexafluorophosphate
-
 Neurospora crassa cellobiono-1,5-lactone + ferricenium hexafluorophosphate
-
 ?
1.1.99.18 D-galactose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 D-glucose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 D-mannose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 D-xylose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 lactose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 maltose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 maltotetraose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 maltotriose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 mannopentaose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 additional information isoform CDH IIA exhibits 4 to 5times higher apparent catalytic constants (kcat and Km values) than isoform CDH IIB for most tested carbohydrates Neurospora crassa ?
-
 ?
1.1.99.18 xylobiose + 1,4-benzoquinone
-
 Neurospora crassa 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 xylotriose + 1,4-benzoquinone
-
 Neurospora crassa ? + reduced 1,4-benzoquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 85000, isoform CDH IIA, SDS-PAGE Neurospora crassa
1.1.99.18 ? x * 85800, isoform CDH IIB, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 CDH IIA isoform Neurospora crassa
1.1.99.18 CDH IIB isoform Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.4
-
 maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1
-
 maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.4
-
 maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.5
-
 D-galactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.5
-
 D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.8
-
 maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.9
-
 D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2.2
-
 maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2.3
-
 xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.5
-
 maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.6
-
 D-galactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 4.3
-
 D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 4.7
-
 D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 5.8
-
 xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 6
-
 xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 7.4
-
 Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 8.1
-
 D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 10.2
-
 cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 11.4
-
 cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 11.6
-
 cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 11.8
-
 lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 12.4
-
 cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 19.8
-
 Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 45.4
-
 xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 45.8
-
 cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 46
-
 cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 46.6
-
 cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 49.3
-
 lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 49.7
-
 cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 55
-
 D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 5
-
 using 2,6-dichlorophenolindol as electron donor Neurospora crassa
1.1.99.18 7
-
 using 1,4-benzoquinone as electron donor Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.18 3.7 8 more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor Neurospora crassa
1.1.99.18 3.8 6.5 more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor Neurospora crassa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.18 Neurospora crassa isoform CDH IIB, isoelectric focusing
-
 4.9
1.1.99.18 Neurospora crassa isoform CDH IIA, isoelectric focusing
-
 5.1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 0.0003
-
 D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.0004
-
 D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.0005
-
 D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.0012
-
 D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.014
-
 D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.015
-
 D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.023
-
 maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.053
-
 maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.061
-
 maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.506
-
 Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.8
-
 maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.88
-
 Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 1.02
-
 maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2
-
 xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2.74
-
 maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2.8
-
 xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 4.45
-
 xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 12.8
-
 xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 133
-
 lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 170
-
 lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 189
-
 cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 216
-
 cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 226
-
 cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 238
-
 cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 238
-
 cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 278
-
 cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 508
-
 cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 527
-
 cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa