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Literature summary extracted from

  • Chen, H.P.; Chow, M.; Liu, C.C.; Lau, A.; Liu, J.; Eltis, L.D.
    Vanillin catabolism in Rhodococcus jostii RHA1 (2012), Appl. Environ. Microbiol., 78, 586-588.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.67 cloned into the pET-28a expression vector and transformed into Escherichia coli Rhodococcus jostii
1.14.13.82 expressed in Escherichia coli Rhodococcus jostii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.82 vanillate + O2 + NADH + H+ Rhodococcus jostii
-
 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Rhodococcus jostii Q0SCE7
-
-
1.14.13.82 Rhodococcus jostii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.67 partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps Rhodococcus jostii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 vanillin + NAD+ + H2O vdh mutant Rhodococcus jostii RHA045 is unable to grow on 1 mM vanillin as the sole organic substrate but grows on 1 mM vanillate with kinetics similar to that of RHA1, mutant rescue by vdh harbouring pTipvdh plasmid Rhodococcus jostii vanillate + NADH + 2 H+
-
 ?
1.14.13.82 vanillate + O2 + NADH + H+
-
 Rhodococcus jostii 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.67 ro02986
-
 Rhodococcus jostii
1.2.1.67 vanillin dehydrogenase
-
 Rhodococcus jostii
1.2.1.67 VDH
-
 Rhodococcus jostii
1.14.13.82 VanA vanillate O-demethylase reductase subunit Rhodococcus jostii
1.14.13.82 vanillate O-demethylase
-
 Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.82 NADH
-
 Rhodococcus jostii

Expression

EC Number Organism Comment Expression
1.14.13.82 Rhodococcus jostii during growth on vanillin or vanillate, the gene vanA is 210fold and 1200fold upregulated, respectively up

General Information

EC Number General Information Comment Organism
1.2.1.67 metabolism enzyme is involved in lignin degradation because one degradation product of lignin is vanillin Rhodococcus jostii